Table 1.
Enrichment for Genes Expressed in Hypothalamic Calcr and LepRb Neurons and Alterations in Calcr Neuron Gene Expression Between Control and LepRbCalcrKO Mice
| Gene |
Enrichment (Fold)
|
Fold Change (Calcr)
|
|
|---|---|---|---|
| Calcr | LepRb | LepRbCalcrKO/Control | |
| Fkbp6 | 1.54 | 1.44 | 4.31 |
| Slc25a31 | 0.76 | 1.46 | 3.61 |
| BC002163 | 1.71 | 1.52 | 2.21 |
| Abcb1a | 0.55 | 4.27 | 2.02 |
| Trpa1 | 0.93 | 1.32 | 1.70 |
| Krt27 | 2.32 | 3.29 | 1.54 |
| Rspo4 | 1.19 | 2.62 | 1.51 |
| Dnajc22 | 1.11 | 0.60 | 1.50 |
| Npy | 10.62 | 21.54 | 1.44 |
| Agrp | 11.59 | 41.89 | 0.99 |
| Calcr | 8.62 | 4.00 | 1.12 |
| Sst | 5.49 | 1.70 | 0.97 |
| Ghrh | 3.73 | 8.96 | 1.08 |
| Klf14 | 3.13 | 4.55 | 0.66 |
| Rab3b | 2.04 | 1.58 | 0.66 |
| Gm5177 | 1.94 | 0.53 | 0.65 |
| Cartpt | 1.52 | 9.38 | 0.64 |
| Serpina3n | 1.79 | 7.35 | 0.60 |
| Skor2 | 8.86 | 1.21 | 0.60 |
| Gstm3 | 3.85 | 1.92 | 0.58 |
| Ndufs5 | 1.60 | 1.44 | 0.54 |
| Procr | 3.21 | 4.99 | 0.51 |
| Serpina3i | 4.13 | 10.59 | 0.45 |
TRAP was performed on CalcreGFP-L10a and LepRbCalcrKO mice; the resultant TRAP and supernatant mRNA was subjected to RNA-seq. Shown are enrichment values for Calcr neurons and for LepRb neurons [from (25)] in Control (untreated or vehicle-treated) mice, along with changes in gene expression in Calcr neurons between Control and LepRbCalcrKO mice for genes found to be differentially expressed, along with other genes of interest. Note that not all genes shown were enriched in Calcr neurons in Control mice; genes shown that were not enriched in Control Calcr neurons were enriched in LepRbCalcrKO Calcr neurons.