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. Author manuscript; available in PMC: 2018 Apr 6.
Published in final edited form as: Immunohorizons. 2017 Aug 15;1(6):101–108. doi: 10.4049/immunohorizons.1700024

FIGURE 3. CCR2 is partially responsible for regulating Ly6C+ monocyte migration from the vasculature to the interstitium after OVA-IC sensitization and lung eosinophilia after allergen challenge.

FIGURE 3

α-OVA serum (OVA-IC) was administered i.v. to naive mice, whereas control mice received either (A) PBS or (B) α-OVAdepl. The next day the mice were challenged i.t. with (A) OVA-FITC or (B) OVA. (A) After 18 h the mice were sacrificed. Minutes prior to sacrifice, the mice received an i.v. injection of anti-CD45 to allow for discrimination between cells in the vasculature and interstitium. Percentage and number of monocytes in the extravascular space are plotted. (B) On days 1, 8, 9, and 10 the mice were challenged i.t. with OVA. On day 11 the mice were sacrificed. Airway inflammation was assessed by determining the number of eosinophils and CD4+ T cells in the BAL by flow cytometry. The data represent the combined data from at least two independent experiments with a total of at least three mice analyzed per group. Data represent the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. ns, not significant.