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. Author manuscript; available in PMC: 2018 Apr 6.
Published in final edited form as: Immunohorizons. 2017 Aug 15;1(6):101–108. doi: 10.4049/immunohorizons.1700024

FIGURE 5. IL-33 upregulates chemokines in the lungs, including monocyte chemoattractants.

FIGURE 5

IL-33−/− mice received i.t. rIL-33 (n = 5) or PBS (n = 4). The mice were sacrificed 6 h later and RNA was purified from total lung homogenate. cDNA was prepared and pooled for each group. A qPCR array was used to assess transcript abundance of genes encoding chemokines and chemokine receptors. Relative expression was calculated using the ΔΔCT method as compared with the housekeeping genes Gusb and Hsp90ab1. Inset: qPCR for Ccl2, Ccl7, and Ccl22 was performed on individual mouse cDNA samples generated from mouse lung homogenates. Chemokine transcripts were normalized to the housekeeping gene Hprt. Dotted line represents expression levels of PBS-treated mice. rIL-33–treated mice (n = 5) were compared with PBS-treated mice (n = 4). #p < 10−10.