Figure 3.

Co-expression of immune suppressive receptors on T cells. (A) Treatment schedule for the evaluation of immunological parameters in single dose irradiation experiments, percentage of intratumoural CD8⁺PD-1⁺ cells of the CD45⁺ cells and median percentages of PD-1⁺ and PD-1⁻ within the CD8⁺ T cell population. (B) Representative FACS histogram and dotplot, percentage of intratumoural CD4⁺PD-1⁺ cells and CD4⁺CD25⁺FoxP3⁺ T cells of CD45⁺ cells in different treatment groups and median percentages of PD-1⁺ and PD-1⁻ or CD25⁺FoxP3⁺ and CD25⁺FoxP3⁻ within the CD4⁺ T cell population. (C) Treatment schedule for the evaluation of immunological parameters in fractionated irradiation experiments. (D) Quantification of CD8⁺ T cells of gated CD45⁺ tumour infiltrating cells, FACS histogram of PD-1 expression on tumour infiltrating CD8⁺ T cells and quantification of CD8⁺PD-1⁺ cells. (E) Quantification of CD4⁺ T cells of gated CD45⁺ tumour infiltrating cells, FACS histogram of PD-1 expression on tumour infiltrating CD4+ T cells and quantification of CD4⁺PD-1⁺ cells. NTM: Normalized to Mode. *p<0.05, **p<0.01. Bars represent median.