Figure 6.

Specific cytokine production and cytotoxic activity of CD8⁺ and CD8⁺CD44⁺CD127⁺ T cells from mice with immunological memory. (A) Percent of CD8⁺ and (B) CD8⁺CD44⁺CD127⁺ T cells after co-culture of C51 or 4T1 (unspecific antigens) tumor cells with immune cells from responders (mice cured by 10Gy+L19-IL2 with tumor rejection after re-challenge), non-responders (mice cured by 10Gy+L19-IL2 with tumor uptake after re-challenge) and naive mice. (C) Percent of INFγ-, TNFα-, Granzyme B- (GZM B) and CD107a-postive CD8⁺ T cells after co-culture of C51 or 4T1 tumor cells with immune cells from responders, non-responders and naive mice. (D) Percent of INFγ-, TNFα-, Granzyme B- (GZM B) and CD107a-postive CD8⁺CD44⁺CD127⁺ T cells after co-culture of C51 or 4T1 tumor cells with immune cells from responders. **p < 0.01, *p < 0.05, #0.05 < p < 0.1. Bars represent median.