Figure 3.

Frequency dependence of Ca²⁺ transient responses to EFS. A, Summary data showing the excitatory effects of EFS (1 Hz for 5 s) on Ca²⁺ transients in ICC-DMP during the final 3 s of EFS and during the poststimulus period (5 s following termination of EFS). Ca²⁺ transient parameters shown include: frequency (s⁻¹), amplitude (ΔF/F₀), duration (FDHM), and spatial spread (μm) of Ca²⁺ transients. B, Summary data showing the effects of EFS (5 Hz; 5 s) on Ca²⁺ transient parameters. C, Summary data showing the effects of EFS (20 Hz; 5 s) on Ca²⁺ transient parameters. D, Percentage (%) change of Ca²⁺ transient firing frequency at all frequencies of EFS tested (1–20 Hz; net percentage change normalized to control) during the final 3 s of EFS and during the poststimulus period. E, Note the frequency-dependent effects of EFS on Ca²⁺ transient responses. Summary data in all panels shows the include 5 s before EFS, the final 3 s during EFS and 5-s post-EFS; ns = p > 0.05, *p < 0.05, **p < 0.01.