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. 2018 Mar 27;9(23):16489–16500. doi: 10.18632/oncotarget.24740

Figure 4. TpeLGTD translocated into the cytosol of host cells by electroporation is sufficient to inactivate Ras.

Figure 4

(A) HCT-116 cells were electroporated in the presence of TpeLGTD and plated for 2 h before lysis. Rasnon-gluc., pERK, and tubulin were probed and a representative blot of three independent experiments is shown. (B) Statistical analysis of phosphorylated ERK following electroporation treatment as presented in A. (C) Capan-2 cells were electroporated in the presence of TpeLGTD and plated for 2 h before lysis. Rasnon-gluc., pERK, and tubulin were probed and a representative blot of three independent experiments is shown. (D) Statistical analysis of phosphorylated ERK following electroporation treatment as presented in C.