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. Author manuscript; available in PMC: 2018 Apr 13.
Published in final edited form as: Chembiochem. 2017 Sep 22;18(21):2099–2103. doi: 10.1002/cbic.201700391

Table 2.

Kinetic parameters for aromatic halogenation catalyzed by RebH and H-16-F.a

FDH kcat (min−1) Km (μM) kcat/Km (min−1 μM−1)
RebH 1.3 1.2 1.06
H-16-F 1.2 1.8 0.66
a

90-160 μM L-tryptophan, 0.5 μM FDH, 0.5 μM reductase, 9 U mL−1 GDH, 10 mM NaCl, 20 mM glucose, 100 μM NAD and FAD, 25 mM HEPES buffer pH = 7.4, 25 °C, 75 μL final reaction volume. Reactions were quenched with MeOH 5-20 minutes after reaction initiation. 0.5 mM phenol was added as an internal standard, and reactions were analyzed by HPLC.