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Fluorescence activated cell sorting for hADAR1d 5’ binding loop library. A) Top: cells expressing the yeGFP reporter and hADAR1d E912A were used to define background fluorescence and above-background fluorescence categories. Bottom: parameters corresponding to each sorting gate. B) Top: cells expressing the yeGFP reporter and hADAR1d loop library were sorted into different categories based on different levels of fluorescence. Bottom: parameters corresponding to each sorting gate.
