Fig. 2.

C. albicans vps4 mutants lack extracellular Sap4-6p. C. albicans cells were grown in RPMI at 37°C in vitro, conditions which are known to induce expression of Sap4p, Sap5p, and Sap6p. Western blotting of culture supernatants from control strain CAF-2, the vps4Δ mutant, and VPS4 reintegrant strain indicates that the vps4Δ mutant fails to produce extracellular Sap4-6p. Anti-Sap2p antibodies were used as a negative control in these growth conditions, and no extracellular Sap2p was detected in any strain (data not shown)