FIGURE 1.

RNA interactome screens identify candidate endoplasmic reticulum RNA-binding proteins (RBPs). (A) Proteomic interactor screens identify 170 poly(A) RNA-binding proteins (mRNPs) in canine pancreas rough microsomes and 21 candidate ER integral membrane mRNPs (Jagannathan et al. 2014a). A tabulated listing of 14 of the 21 candidates is provided. (B) Venn diagrams of ER-associated integral membrane mRNA-binding proteins identified in HeLa cells, HEK293 cells, and mouse embryonic stem cells (mESC) (Baltz et al. 2012; Castello et al. 2012; Kwon et al. 2013). The candidate mRBPs identified in the canine rough microsome screen in A are underlined. (C) Schematic of experimental protocol for identifying candidate RNA-binding activity in living cells by UV cross-linking and immunoprecipitation (CLIP). A representative phosphorimage depicting GFP-Sec61β and GFP-AEG-1 RNA complexes’ formation by UV cross-linking is depicted. The empty vector transfection condition is included to depict background labeling.