Figure 5. Abnormal spontaneous Ca²⁺ activities in vivo from Mecp2 null astrocytes.

(A) Representative GCaMP6s image showing two astrocytes in vivo. The somas and the processes could be clearly identified. Scale bars = 10 μm. (B) Representative ΔF/F₀ traces showing the spontaneous intracellular Ca²⁺ activity in the soma of astrocytes in vivo from wild type and Mecp2 null astrocytes. (C) Quantification of the frequency (left) and the full width at half maximum (FWHM, right) of the astrocytic Ca²⁺ oscillations in vivo. The bar graphs in this figure show the mean ±s.e.m. ***p<0.001.

Figure 5—figure supplement 1. Co-staining of MeCP2 and GCaMP6 in cortex from WT (A) and MeCP2^flox/y mice (B) that received AAV8-hGFAP-mCherry-Cre injection.

Note that all of the GFP positive cells from WT mice (marked by white arrows, (A) are positive for MeCP2, while all of the GFP positive cells from MeCP2^flox/y mice (marked by white arrowheads, (B) are negative for MeCP2.

Figure 5—figure supplement 2. Quantification of spontaneous Ca²⁺ activity in astrocytic processes from live WT and Mecp2 null mice.

(A) GCaMP6 fluorescence traces in astrocytic processes from WT and Mecp2 null mice. (B) Quantification of the frequency and full width at half maximum (FWHM) of the spontaneous Ca²⁺ activity in astrocytic processes. ***p<0.001. Please see Table 2 for quantification of the amplitude.