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. Author manuscript; available in PMC: 2019 Jun 1.
Published in final edited form as: Glia. 2017 Dec 8;66(6):1200–1212. doi: 10.1002/glia.23271

Figure 4. Glucose metabolism affects microglial activation and ROS production at the transcriptional level.

Figure 4

(A) Immunostaining for CD11b identifies activated microglia in rat hippocampus. CD11b expression was increased 24 hours after intraperitoneal injection with LPS (10 mg/kg). The increase was attenuated by co-injection with the glycolytic inhibitor 2-deoxyglucose (2DG; 100mg/kg). Scale bar = 100 μm. ** p < 0.01, n = 6. (B) Immunostaining for Iba1 and iNOS identify activated microglia in mouse hippocampal slice cultures after 24 hours incubation with LPS (10 μg/ml) or LPS +2DG (1 mM). Scale bar = 100 μm; n ≥ 3, * p < 0.05. Culture medium for contained 6 mM glutamine and 5 mM glucose. (C) Effects of 1mM 2DG on LPS - induced iNOS transcript and protein expression in microglial cultures. n = 5; * p < 0.05, ** p < 0.01. Data are means ± s.e.m. Figure adapted from (Shen et al. 2017).