Abstract
A B‐cell line having translocations of chromosome 14 at break band q11 (the assigned locus of the α‐chain gene of the T‐cell antigen receptor) and chromosome 3 at break band p25 (the assigned locus of the c‐raf‐1 oncogene) was established from peripheral blood leukocytes of an adult T‐cell leukemia (ATL) patient. The same chromosome 14 aberration at break band q11 and chromosome 3 aberration at break band p25 were also found in fresh T‐cell leukemia cells. The B‐cell line is surface immunoglobulin (sIg)+, immunoglobulin gene rearrangement+, ATL‐specific antigen (ATLA)+, HTLV‐1 proviral genome+, Epstein‐Barr virus (EBV)‐associated nuclear antigen (EBNA)+ and the EBV DNA genome+. The fresh T‐leukemic cells were T‐cell receptor gene rearrangement+, the HTLV‐1 proviral genome+ and EBV DNA genome−.
Keywords: ATL, B‐cell line, HTLV‐1, EBNA, Chromosome 14
Full Text
The Full Text of this article is available as a PDF (384.4 KB).
REFERENCES
- 1).Uchiyama , T. , Yodoi , J. , Sagawa , K. , Takatsuki , K. and Uchino , H.Adult T cell leukemia: clinical and hematologic features in 16 cases . Blood , 50 , 481 – 492 ( 1977. ). [PubMed] [Google Scholar]
- 2).Poiesz , B. J. , Ruscetti , F. W. , Gazdar , A. F. , Bunn , P. A. , Minna , J. P. and Gallo , R. C.Detection and isolation of type C retrovirus particles from fresh and cultured lymphocytes of a patient with cutaneous T‐cell lymphoma . Proc. Natl. Acad. Sci. USA , 77 , 7415 – 7419 ( 1980. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
- 3).Hinuma , Y. , Nagata , K. , Hanaoka , M. , Nakai , M. , Matsumoto , T. , Kinoshita , K. , Shirakawa , S. and Miyoshi , I.Adult T‐cell leukemia: antigen in an ATL cell line and detection of antibodies to the antigen in human sera . Proc. Natl. Acad. Sci. USA , 78 , 6476 – 6480 ( 1981. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
- 4).Zech , L. , Gahrton , G. , Hammarström , L. , Juliusson , G. , Mellstedt , H. , Robért , K. H. and Smith , C. I. E.Inversion of chromosome 14 marks human T‐cell chronic lymphocytic leukemia . Nature , 308 , 858 – 860 ( 1984. ). [DOI] [PubMed] [Google Scholar]
- 5).Ueshima , Y. , Rowley , J. D. , Variakojis , D. , Winter , J. and Gordon , L.Cytogenetic studies on patients with chronic T cell leukemia/lymphoma . Blood , 63 , 1023 – 1038 ( 1984. ). [PubMed] [Google Scholar]
- 6).Hecht , F. , Morgan , R. , Hecht , B. K‐M. and Smith , S. D.Common region on chromosome 14 in T‐cell leukemia and lymphoma . Science , 226 , 1445 – 1447 ( 1984. ). [DOI] [PubMed] [Google Scholar]
- 7).Sadamori , N. , Nishino , K. , Kusano , M. , Tomonaga , Y. , Tagawa , M. , Yao , E. , Sasagawa , I. , Nakamura , H. and Ichimaru , M.Significance of chromosome 14 anomaly at band 14q11 in Japanese patients with adult T‐cell leukemia . Cancer , 58 , 2244 – 2250 ( 1986. ). [DOI] [PubMed] [Google Scholar]
- 8).Dubé , I. D. , Raimondi , S. C. , Pi , D. and Kalousek , D. K.A new translocation, t(1O;14)(q24;q11), in T cell neoplasia . Blood , 67 , 1181 – 1184 ( 1986. ). [PubMed] [Google Scholar]
- 9).Clare , N. , Boldt , D. , Messerschmidt , G. , Zeltzer , P. , Hansen , K. and Manhoff , L.Lymphocyte malignancy and chromosome 14: structural aberrations involving band q11 . Blood , 67 , 704 – 709 ( 1986. ). [PubMed] [Google Scholar]
- 10).Smith , S. D. , Morgan , R. , Link , M. P. , McFall , P. and Hecht , E.Cytogenetic and immunophenotypic analysis of cell lines established from patients with T cell leukemia/lymphoma . Blood , 67 , 650 – 656 ( 1986. ). [PubMed] [Google Scholar]
- 11).Miyamoto , K. , Tomita , N. , Ishii , A. , Miyamoto , N. , Nonaka , H. , Kondo , T. , Sugihara , T. , Yawata , Y. , Tada , S. , Tsubota , T. , Kitajima , K. and Kimura , I.Specific abnormalities of chromosome 14 in patients with acute type of adult T‐cell leukemia/lymphoma . Int. J. Cancer , 40 , 461 – 468 ( 1987. ). [DOI] [PubMed] [Google Scholar]
- 12).Miyamoto , K. , Tomita , N. , Ishii , A. , Nishizaki , T. and Togawa , A.Establishment and characterization of adult T‐cell leukemia virus‐containing B‐cell lines derived from peripheral blood of adult T‐cell leukemia patients . Gann , 75 , 655 – 659 ( 1984. ). [PubMed] [Google Scholar]
- 13).Yamamoto , N. , Matsumoto , T. , Koyanagi , Y. , Tanaka , Y. and Hinuma , Y.Unique cell lines harbouring both Epstein‐Barr virus and adult T‐cell leukaemia patients . Nature , 299 , 367 – 369 ( 1982. ). [DOI] [PubMed] [Google Scholar]
- 14).Longo , D. L. , Gelmann , E. P. , Cossman , J. , Young , R. A. , Gallo , R. C. , O'Brien , S. J. and Matis , L. A.Isolation of HTLV‐transformed B‐lymphocytes clone from a patient with HTLV‐associated adult T‐cell leukaemia . Nature , 310 , 505 – 507 ( 1984. ). [DOI] [PubMed] [Google Scholar]
- 15).Mann , D. L. , DeSantis , P. , Mark , G. , Pfeifer , A. , Newman , M. , Gibbs , N. , Popovic , M. , Sarngadharan , M. G. , Gallo , R. C. , Clark , J. and Blattner , W.HTLV‐1‐associated B‐cell CLL: indirect role for retrovirus in leukemogenesis . Science , 236 , 1103 – 1106 ( 1987. ). [DOI] [PubMed] [Google Scholar]