Figure 4.
Heat-induced punctate structures of ATI3-ATG8 complexes. Agrobacterium cells containing the complementing ATG8A-N-YFP and ATI3A-C-YFP BiFC constructs were infiltrated into N. benthamiana leaves for co-expression. One d after the infiltration, tobacco plants were treated at 42°C for 3 h and complemented BiFC signals were observed by confocal fluorescence microscopy. Increased punctate structures of complemented ATG8A-N-YFP-ATI3A-C-YFP BIFC signals were observed after treatment at 42°C. No BiFC signals were observed from co-expression of ATG8A-N-YFP and ATI3AW260A-C-YFP at either 24°C or 42°C. Bar: 20 μm.