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. 2018 Apr 6;9(26):18454–18479. doi: 10.18632/oncotarget.24862

Figure 3. ONC201 inhibits mitochondrial respiration.

Figure 3

(A) OCR/ECAR measurement of MB231 cells treated with either DMSO (Ctl.) or ONC201 (5μM) in glucose or galactose medium for 24 h. OCR/ECAR were measured 5 times with 5 replicates for each condition. Data shown here is one representative result of 4 independent experiments. (B) Long time-course OCR measurement of MB231 cells. Drugs was injected via ports of the XF analyzer cartridge, with 5 replicates per each drug. OCR was measured every ~11 minutes and followed up to 1,200 minutes (20 h). Data is shown as mean +/− SEM. (C) OCR measurement with freshly isolated mitochondria from MB231 cells. Drugs were injected via ports of the XF analyzer cartridge. OCR was measured every ~3 minutes, 3 times, before and after injection (total ~20 minutes), with 4 replicates per each drug. Data is shown as mean +/− SEM of one representative result of 3 independent experiments.