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Knock down efficiency of HNF-1β (A), Claspin (B), and USP28 (C) siRNA. Western blot analysis assays revealed that the protein expression levels of HNF-1β (A), Claspin (B), and USP28 (C) were significantly decreased in the TOV21G si-HNF-1β, si-Claspin and si-USP28 groups when compared with the TOV21G si-Ctl group, with β-actin protein as the internal control. Knock-down was efficient in every experiment, resulting in a 70-85% reduction of the corresponding protein.
