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. 2018 Apr 3;9(25):17512–17522. doi: 10.18632/oncotarget.24776

Figure 6. Effect of USP28 on the Claspin and p-Chk1 proteins expression after bleomycin.

Figure 6

(A) Western blot showing the protein levels of Claspin and p-Chk1 in TOV21G cells transfected with si-USP28 or si-Ctl. Knockdown of endogenous USP28 led to a decrease in Claspin and p-Chk1 expression after bleomycin treatment. si-Ctl and si-USP28 samples were loaded on the same gel. (B) Densitometry results of immunoblots for Claspin. (C) Densitometry results of immunoblots for p-Chk1. Data are represented as fold-difference compared with si-Ctl after correcting for loading (by β-actin expression). Figure shows representative images from at least three independent Western blot assays.