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. 2018 Apr 3;9(25):17512–17522. doi: 10.18632/oncotarget.24776

Figure 8. Viability of TOV21G cells following HNF-1β, Claspin or USP28 siRNA transfection.

Figure 8

(A) After the transfection with Claspin, HNF-1β and control siRNAs was repeated twice (at day 1 and day 3), bleomycin (42 μM) was added to the TOV21G cells at day 4 and further incubated for 48 hours. (B) In a separate experiment, TOV21G cells were transfected with USP28, HNF-1β and control siRNAs at day 1 and treated with bleomycin at day 3. After 48 hours incubation, the cytotoxicity was determined by the trypan blue exclusion test. Cell viability (%) = living cells / total cells × 100%. The viability of TOV21G cells was evaluated using a trypan blue assay. Each condition was done in duplicates.