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. 2018 Feb 1;10(3):431–443. doi: 10.1080/19420862.2018.1426422

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© 2018 GigaGen Inc. Published with license by Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 3. — scFv libraries subjected to FACS for IL-21R. Staining for c-Myc (AF488) was used to differentiate yeast cells that express scFv from yeast cells that do not express scFv (x-axis). Staining for biotinylated IL-21R (PE) was used to identify yeast that express scFv that bind to the antigen (y-axis). The Fc negative control was used to set gates that are used to capture yeast clones that express scFv and which also bind antigen (upper right corner of the FACS plot). Gates for yeast selection are indicated by the quadrangle in the upper right corner of each FACS plot. The percentage in each quadrangle (red type) indicates the proportion of yeast that expressed c-Myc and fell within the gate. A vertical dotted line (black) indicates the gate used to determine the number of yeast that express scFv (c-Myc+). (A) 1^st and 2^nd sort FACS data for the natively paired scFv library. (B) 1^st and 2^nd sort FACS data for the randomly paired scFv library.