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Japanese Journal of Cancer Research : Gann logoLink to Japanese Journal of Cancer Research : Gann
. 1988 Mar;79(3):314–319. doi: 10.1111/j.1349-7006.1988.tb01593.x

The Promoting Effects of Food Dyes, Erythrosine (Red 3) and Rose Bengal B (Red 105), on Thyroid Tumors in Partially Thyroidectomized N‐Bis(2‐hydroxypropyl)‐ nitrosamine‐treated Rats

Yoshio Hiasa 1, Masato Ohshima 1, Yoshiteru Kitahori 1, Noboru Konishi 1, Taketo Shimoyama 1, Yasuhiro Sakaguchi 1, Hiroyuki Hashimoto 1, Shigenori Minami 1, Yuzuru Kato 2
PMCID: PMC5917475  PMID: 2836348

Abstract

The effects of erythrosine (Red 3), rose bengal B (Red 105) and thyroidectomy on the development of thyroid tumor were examined in male Wistar rats treated with N‐bis(2‐hydroxypropyl)nitrosamine (DHPN). Red 3 and Red 105 were used at 4% in the basal diet and were administered for 19 weeks from week 2 to 20. Thyroidectomy was performed by resection of the left lobe at week 4. Single injection of DHPN was performed intraperitoneally at 280 mg per 100 g body weight at the beginning of the experiment. Red 3 and Red 105 significantly promoted the development of thyroid tumors in thyroidectomized rats given DHPN, but had no significant effect in non‐thyroidectomized rats. The incidence of thyroid tumors was 91% in rats with partial thyroidectomy, Red 3 and DHPN, 100% in rats with partial thyroidectomy, Red 105 and DHPN, and 64% in rats with partial thyroidectomy and DHPN. Serum TSH was 5.5±3.1 ng/ml in rats with partial thyroidectomy, Red 3 and DHPN, 2.1 ± 2.2 ng/ml in rats with partial thyroidectomy, Red 105 and DHPN, and 1.5±0.5 ng/ml in rats with partial thyroidectomy and DHPN.

Keywords: N‐Bis(2‐hydroxypropyl)nitrosamine, Thyroid tumor, Promoter, Erythrosine (Red 3), Rose bengal B (Red 105)

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