Abstract
A moderately differentiated tubular adenocarcinoma of human stomach, named SY86B, was successfully transplanted subcutaneously to nude mice of different genetic backgrounds (BALB/CA/PBI‐nu, C57BL/6J.615/PBI‐nu and ICR‐BALB/CA/PBI‐nu). The tumor has been passaged for 13 generations and the transplantability was 100%. The SY86B cells retained the capacity of invasive and metastatic growth in the nude mice and showed a high rate of metastasis to the regional lymph nodes and to such distant organs as the lungs, liver and pancreas. The overall rate of metastasis was 77.7%. The species of the nude mice, their age and sex apparently did not significantly affect the occurrence of metastasis. Tumor‐bearing time and the aggressive character of the tumor cells themselves appeared important for the genesis of metastasis. This experimental model can provide a new approach to basic and clinical studies of cancer metastasis.
Keywords: Metastasis, Nude mouse, Human gastric carcinoma
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REFERENCES
- 1. ) Sharkey , F. E. and Fogh , J.Metastasis of human tumors in athymic nude mice . Int. J. Cancer , 24 , 733 – 738 ( 1979. ). [DOI] [PubMed] [Google Scholar]
- 2. ) Kyriazis , A. P. , Kyriazis , A. A. , McCombs , W. B.III , and Kereiakes , J. A.Biological behavior of human malignant tumors grown in the nude mouse . Cancer Res. , 41 , 3995 – 4000 ( 1981. ). [PubMed] [Google Scholar]
- 3. ) Rygaard , T. “ The Nude Mouse in Experimental and Clinical Research ,” pp. 95 – 109 ( 1978. ). Academic Press Inc. , New York . [Google Scholar]
- 4. ) Giovanella , B. C. , Yim , S. O. , Morgan , A. C. , Stehlin , J. S. and William , L. J.Jr.Metastasis of human melanomas transplanted in “nude” mice . J. Natl. Cancer Inst. , 50 , 1051 – 1053 ( 1973. ). [DOI] [PubMed] [Google Scholar]
- 5. ) Hata , J. , Ueyama , Y. , Tamaoki , N. , Furukawa , T. and Morita , K.Human neuroblastoma serially transplanted in nude mice and metastases . Cancer , 42 , 468 – 473 ( 1978. ). [DOI] [PubMed] [Google Scholar]
- 6. ) Ezaki , T. , Nakatani , K. , Miyagi , N. , Sakamoto , K. , Takahashi , S. , Emi , Y. , Shiratori , T. and Konishi , Y.Establishment of an α‐fetoprotein‐producing human gastric carcinoma in nude mice . Gann , 74 , 870 – 877 ( 1983. ). [PubMed] [Google Scholar]
- 7. ) Naomoto , Y. , Tanaka , N. , Kondo , H. and Orita , K.An experimental model of pulmonary metastasis of a human colon cancer in nude mice . Igaku-no-Ayumi , 139 , 343 – 344 ( 1986. ) ( in Japanese ). [Google Scholar]
- 8. ) Takao , S. , Nishi , M. , Maenohara , S. , Nomura , H. and Aikou , T.Studies on transplantable and metastasizing AFP‐producing human gastric cancer in nude mice . Igaku-no-Ayumi , 128 , 727 – 728 ( 1984. ) ( in Japanese ). [Google Scholar]
- 9. ) Li , H. and Zhang , Y‐C.Establishment of human gastric carcinoma model of nude mice and its biological characteristics . J. China Med. Univ. , 16 ( 2 ), 6 – 10 ( 1987. ) ( in Chinese ). [Google Scholar]
- 10. ) Katano , M. , Saxton , R. E. , Cochran , A. J. and Irie , R. F.Establishment of an ascitic human melanoma cell line that metastasizes to lung and liver in nude mice . J. Cancer Res. Clin. Oncol. , 108 , 197 – 203 ( 1984. ). [DOI] [PubMed] [Google Scholar]
- 11. ) Kerbel , R. S. , Man , M. S. and Dexter , D.A model of human cancer metastasis; extensive spontaneous and artificial metastasis of a human pigmented melanoma and derived variant sublines in nude mice . J. Natl. Cancer Inst. , 72 , 93 – 108 ( 1984. ). [DOI] [PubMed] [Google Scholar]