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Japanese Journal of Cancer Research : Gann logoLink to Japanese Journal of Cancer Research : Gann
. 1988 Oct;79(10):1119–1129. doi: 10.1111/j.1349-7006.1988.tb01535.x

Production of Monoclonal Antibodies Directed against Carbohydrate Moieties of Cell Surface Glycoproteins

Shigeyuki Fukui 1, Yoshito Numata 1, Akira Kurosaka 2, Hiroshi Kitagawa 1, Hiroshi Nakada 2, Ikuo Funakoshi 1, Toshisuke Kawasaki 1, Yohei Takahashi 3, Kazuma Hayashi 3, Ikuo Yamashina 1,✉
PMCID: PMC5917637  PMID: 3143700

Abstract

Through the use of a technique for raising monoclonal antibodies, coupled with a solid‐phase radioimmunoassay utilizing immobilized glyeopeptides prepared from the surface membranes of the colorectal cancer cells (LS 180) used for the immunization, carbohydrate‐directed monoclonal antibodies were obtained. One of the monoclonal antibodies, MLS 102, reacted immunohistochemically intensely with the colorectal cancer cell surface and the mutinous glycoproteins secreted by the cancer cells, but only weakly with normal colon tissue. The antigenic determinant recognized by MLS 102 was the carbohydrate moiety of glycoproteins with terminal sialic acid. The antigens defined by other monoclonal antibodies, MLS 103 and 104, were immunohistochemically detected in both normal colonic epithelial and cancer cells. These antibodies seemed to recognize the carbohydrate moieties of both glycoproteins and glycolipids. The method described in this report can be generally applied to raise cell surface carbohydrate‐directed antibodies.

Keywords: Monoclonal antibody, Colorectal cancer, Tumor‐associated antigen, Mucin‐type glycoprotein, Mucin

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