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Japanese Journal of Cancer Research : Gann logoLink to Japanese Journal of Cancer Research : Gann
. 1989 Jan;80(1):59–64. doi: 10.1111/j.1349-7006.1989.tb02245.x

Lysis of Human Leukemic Cells by Monocyte‐derived Macrophages Activated with Interferon‐γ and Interleukin‐2

Tokio Kakita 1, Masataka Sasada 2,✉, Toshinori Moriguchi 1, Toshiro Nishimura 1, Kohkichi Yamamoto 1, Haruto Uchino 1
PMCID: PMC5917684  PMID: 2496059

Abstract

Cytolysis of leukemic cells by peripheral blood‐derived macrophages was examined by means of an in vitro111In release assay. Monocytes prepared on culture dishes lyse YK‐M2. However, when monocytes were cultured in vitro and transformed into macrophages, they lost most of their lytic activity. The addition of human recombinant interleukin‐2 (rIL‐2) on day 5 in culture enhanced the lytic activity significantly. Similarly, treatment of macrophages with human recombinant interferon gamma (rIFN‐γ) promoted the lysis of YK‐M2 and K‐562, although the extent of lysis was smaller than that by rIL‐2. Macrophages activated with rIL‐2 and rIFN‐γ also Iysed human leukemic cells. Activated macrophages lysed leukemic cells of acute myelocytic leukemia more than acute lymphocytic leukemia cells. Macrophages derived from the peripheral blood of patients with leukemia were examined for their lytic activity against YK‐M2. The patient's macrophages lysed more YK‐M2 than did control macrophages when they were activated with rIL‐2 and rIFN‐γ. The macrophages of two patients also demonstrated autologous leukemic cell lysis.

Keywords: Macrophage, Recombinant interleukin‐2, Recombinant interferon‐γ, Leukemic cell, Leukemia

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