Abstract
Expression of the c‐myc gene product in urinary bladder cancer was investigated by immunohistochemical staining with anti‐c‐myc monoclonal antibody (mAb) MYC‐1. Positive staining was observed in the cytoplasm, but not in the nucleus in tissues fixed with 10% formalin. On the other hand, positive staining was localized in the nucleus in cryopreserved tissues. Of 34 cryopreserved specimens examined, positive staining with MYC‐1 mAb was observed in 1 of 12 (8.3%) of grade 1 (G1), 12 of 15 (80%) of G2 and 6 of 7 (86%) of G3. Positive staining with Ki‐67 mAb was observed in 2 of 12 (17%) of G1, 12 of 15 (80%) of G2, and 6 of 7 (86%) of G3. These results suggest that tumors with higher nuclear pleomorphism contain more proliferating cells.
Keywords: c‐myc, Immunohistochemistry, Bladder cancer, Nuclear pleomorphism
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REFERENCES
- 1. ) Kelly , K. , Cochran , B. H. , Stiles , C. D. and Leder , P.Cell‐specific regulation of the c‐myc gene by lymphocyte mitogens and platelet‐derived growth factors . Cell , 35 , 603 – 610 ( 1983. ). [DOI] [PubMed] [Google Scholar]
- 2. ) Studzinski , G. P. , Brelvi , Z. S. , Feldman , S. C. and Watt , R. A.Participation of c‐myc protein in DNA synthesis of human cells . Science , 234 , 467 – 470 ( 1986. ). [DOI] [PubMed] [Google Scholar]
- 3. ) Heikkila , R. , Schwab , G. , Wickstrom , E. , Loke , S. L. , Pluznik , D. H. , Watt , R. and Neckers , L. M.A c‐myc antisense oligonucleotide inhibits entry into S phase but not progress from G0 to G1 . Nature , 328 , 445 – 449 ( 1987. ). [DOI] [PubMed] [Google Scholar]
- 4. ) Kinouchi , T. , Saiki , S. , Naoe , T. , Uenaka , A. , Kotake , T. , Shiku , H. and Nakayama , E.Correlation of c‐myc expression with nuclear pleomorphism in human renal cell carcinoma . Cancer Res. , 49 , 3627 – 3630 ( 1989. ). [PubMed] [Google Scholar]
- 5. ) Naoe , T. , Nozaki , N. , Yamada , K. , Okazaki , T. , Nakayama , E. , Kurosawa , Y. and Shiku , H.Diversity of cellular molecules in human cells detected by monoclonal antibodies reactive with c‐myc proteins produced in Escherichia coli . Jpn. J. Cancer Res. , 80 , 747 – 753 ( 1989. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
- 6. ) Gerdes , J. , Schwab , U. , Lemke , H. and Stein , H.Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation . Int. J. Cancer , 31 , 13 – 20 ( 1983. ). [DOI] [PubMed] [Google Scholar]
- 7. ) Yoshida , K. , Hamatani , K. , Koide , H. , Ikeda , H. , Nakamura , N. , Akiyama , M. , Tsuchiyama , H. , Nakayama , E. and Shiku , H.Preparation of anti‐ras Mr 21,000 protein monoclonal antibodies and immunohistochemical analyses on expression of ras genes in human stomach and thyroid cancers . Cancer Res. , 48 , 5503 – 5509 ( 1988. ). [PubMed] [Google Scholar]
- 8. ) Japanese Urological Association and Japanese Pathological Society . “ General Rules for Clinical and Pathological Studies on Bladder Cancer ,” Ed. 1 ( 1980. ). Kanehara Co., Ltd. , Tokyo ( in Japanese ). [Google Scholar]
- 9. ) Donner , P. , Wilke , I. G. and Moelling , K.Nuclear localization and DNA binding of the transforming gene product of avian myelocytomatosis virus . Nature , 296 , 262 – 266 ( 1982. ). [DOI] [PubMed] [Google Scholar]
- 10. ) Alitalo , K. , Ramsay , G. , Bishop , J. M. , Pfeifer , S. O. , Colby , W. W. and Levinson , A. D.Identification of nuclear proteins encoded by viral and cellular myc oncogenes . Nature , 306 , 274 – 277 ( 1983. ). [DOI] [PubMed] [Google Scholar]
- 11. ) Stewart , J. , Evan , G. , Watson , J. and Sikora , K.Detection of the c‐myc oncogene product in colonic polyps and carcinomas . Br. J. Cancer , 53 , 1 – 6 ( 1986. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
- 12. ) Sikora , K. , Evan , G. , Stewart , J. and Watson , J. V.Detection of the c‐myc oncogene product in testicular cancer . Br. J. Cancer , 52 , 171 – 176 ( 1985. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
- 13. ) Iwanaga , T. , Fujita , T. , Tsuchihashi , T. , Yamaguchi , K. , Abe , K. and Yanaihara , N.Immunocytochemical detection of the c‐myc oncogene product in human fetuses . Biomed. Res. , 7 , 161 – 166 ( 1986. ). [Google Scholar]
- 14. ) Fradet , Y. , Cordon‐Cardo , C. , Thomson , T. , Daly , M. E. , Whitmore , W. F. , Jr. , Lloyd , K. O. , Melamed , M. R. and Old , L. J.Cell surface antigens of human bladder cancer defined by mouse monoclonal antibodies . Proc. Natl. Acad. Sci. USA , 81 , 224 – 228 ( 1984. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
- 15. ) Sikora , K. , Chan , S. , Evan , G. , Gabra , H. , Markham , N. , Stewart , J. and Watson , J.c‐myc oncogene expression in colorectal cancer . Cancer , 59 , 1289 – 1295 ( 1987. ). [DOI] [PubMed] [Google Scholar]
- 16. ) Takahashi , T. , Obata , Y. , Sekido , Y. , Hida , T. , Ueda , R. , Watanabe , H. , Ariyoshi , Y. , Sugiura , T. and Takahashi , T.Expression and amplification of myc gene family in small cell lung cancer and its relation to biological characteristics . Cancer Res. , 49 , 2683 – 2688 ( 1989. ). [PubMed] [Google Scholar]