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Japanese Journal of Cancer Research : Gann logoLink to Japanese Journal of Cancer Research : Gann
. 1990 Mar;81(3):225–231. doi: 10.1111/j.1349-7006.1990.tb02554.x

Heterogeneity of Antigen Molecules Recognized by Anti‐tax1 Monoclonal Antibody Lt‐4 in Cell Lines Bearing Human T Cell Leukemia Virus Type I and Related Retroviruses

Yuetsu Tanaka 1,✉, Atsushi Yoshida 1, Yoshinaga Takayama 1, Hajime Tsujimoto 1,2,†, Atsumi Tsujimoto 1,3,‡, Masanori Hayami 4, Hideki Tozawa 1
PMCID: PMC5918036  PMID: 2161813

Abstract

Using a monoclonal antibody, Lt‐4, directed against human T cell leukemia virus type I (HTLV‐I) trans‐activator (tax1) antigen, we examined the expression of tax1 and related antigens in a variety of T cell lines bearing HTLV‐I and related retroviruses, simian T cell leukemia virus type I (STLV‐I) and HTLV‐II, by immunofluorescence and immunoblot assays. Lt‐4 reacted with all HTLV‐I‐bearing cell lines tested and five out of eight simian cell lines bearing STLV‐I, but not with an HTLV‐II‐bearing cell line. Lt‐4 detected 40 kd tax1 antigen molecules in most HTLV‐I‐bearing cell lines except one cell line that expressed 39 kd tax1 antigen. In the STLV‐I‐bearing T cell lines, tax1‐related antigen molecules detected by Lt‐4 were heterogeneous, having molecular weights in the range of 36–41 kd.

Keywords: HTLV‐I, STLV‐I, HTLV‐II, trans‐Activator antigen, Monoclonal antibody

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