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. 1990 Mar;81(3):232–237. doi: 10.1111/j.1349-7006.1990.tb02555.x

A Simple, General Method for Detecting Retroviral RNAs Expressed in Cells

Fumio Harada 1, Yutaka Hirose 1, Motohiro Tanaka 2, Takuma Sasaki 2, Hiroyuki Kamiya 3, Kazunobu Miura 3,†
PMCID: PMC5918037  PMID: 2112525

Abstract

A simple, efficient method has been developed for detecting retroviral RNAs expressed in cells. In this method, total RNAs or poly A+ RNAs extracted from various human cells are separated by electrophoresis and hybridized with synthetic oligonucleotides corresponding to the 3′‐terminal 18 nucleotides of various tRNAs. Genomic and subgenomic RNAs of HTLV‐I and HTLV‐II in virus‐infected cells and of xenotropic murine leukemia virus expressed in human lung cancer cells were easily detected with the tRNAPro‐derived oligonucleotide probe. This technique can be used to search for unidentified retroviruses expressed in human cancer cells and tissues.

Keywords: Retrovirus, Primer binding site, Primer tRNA, Viral RNA, Xenotropic murine leukemia virus

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