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Japanese Journal of Cancer Research : Gann logoLink to Japanese Journal of Cancer Research : Gann
. 1990 Sep;81(9):871–877. doi: 10.1111/j.1349-7006.1990.tb02660.x

Inhibitory Effects of Antioxidants on N‐Bis(2‐hydroxypropyl)nitrosamine‐induced Lung Carcinogenesis in Rats

Ryohei Hasegawa 1,✉, Fumio Furukawa 1, Kazuhiro Toyoda 1, Michihito Takahashi 1, Yuzo Hayashi 1, Masao Hirose 2, Nobuyuki Ito 2
PMCID: PMC5918114  PMID: 2121688

Abstract

Potential second‐stage modifying effects of 8 antioxidants on lung tumorigenesis initiated by N‐bis(2‐hydroxypropyl)nitrosamine (DHPN) were examined in male F344 rats. After an initial 2‐week treatment with DHPN (0.1% in drinking water), rats were administered one of the antioxidants supplemented in the diet for 30 weeks. Although the incidences of lung adenomas were not affected, those of carcinomas were lowered by 2% butylated hydroxyanisole (BHA, 2 rats/20 rats), 1% butylated hydroxytoluene (BHT, 1/20), 0.8% ethoxyquin (EQ, 3/20) and 1%α‐tocopherol (α‐TP, 2/20) treatments as compared to the control level (9/20), while 5% sodium l‐ascorbate (SA), 0.8% catechol (CC), 0.8% resorcinol (RN), and 0.8% hydroquinone (HQ) did not exert any significant effect on incidence. Quantitative analysis of adenomas and carcinomas (numbers and areas of lesions per unit area of lung section) revealed obvious inhibitory effects of SA, CC, and RN as well as BHA, BHT, EQ, and α‐TP. Among the antioxidants, BHT exerted the strongest inhibitory activity. In contrast, DHPN‐induced thyroid tumorigenesis was significantly enhanced by BHT (14/20) and EQ (20/20) treatments (control=5/20). Thus the antioxidants showed opposite effects on lung and thyroid carcinogenesis in the rat.

Keywords: Antioxidant, Lung tumorigenesis, nhibition, Rat

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