Abstract
We have examined the localization of epidermal growth factor (EGF) receptors and E‐cadherins in cultured A431 cells, an epidermoid carcinoma cell line, using a laser scanning fluorescence microscope. The fluorescence signals generated by monoclonal antihodies against EGF receptor and E‐cadherin were localized mainly in the cell‐cell contact sites, about 3 μm in width. When these areas for cell adhesion were scanned perpendicularly, fluorescence was detected from a point 4μ from the cell base for a distance of 8 μm towards the cell surface (about 16 μm from the cell base). These data suggest the subcellular localization of EGF receptors and E‐cadherins in the basolateral membrane of A431 cells.
Keywords: EGF receptor, E‐cadherin, Cell attachment, Laser scanning microscopy
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REFERENCES
- 1. ) Adamson , E.D. , and Rees , A. R . Epidermal growth factor receptors . Mol Cell Biochem. , 34 , 129 – 152 ( 1981. ). [DOI] [PubMed] [Google Scholar]
- 2. ) Shimizu , N.Cell genetic analysis of the receptor systems for bioactive polypeptides . Recept. Recognit., Ser. B , 16 , 109 – 142 ( 1984. ). [Google Scholar]
- 3. ) Nanney , L. B. , Magid , M , Stoscheck , C. M. and King , L. E.Comparison of epidermal growth factor binding and receptor distribution in normal human epidermis and epidermal appendages . J. Inv. Dermatol , 83 , 385 – 393 ( 1984. ). [DOI] [PubMed] [Google Scholar]
- 4. ) Haigler , H. , Ash , J. R , Singer , S. J. and Cohen , S.Visualization by fluorescence of the binding and internaliza‐tion of epidermal growth factor in human carcinoma cells A431 . Proc. Nail. Acad. Sci. USA , 75 , 3317 – 3321 ( 1978. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
- 5. ) Amagai , M. , Ozawa , S. , Ueda , M , Nishikawa , T. , Abe , O. and Shimizu , N.Distribution of EGF receptor expressing and DNA replicating epidermal cells in psoriasis vulgaris andBowenapos;s disease . Br. J. Dermatol , 119 , 661 – 668 ( 1988. ). [DOI] [PubMed] [Google Scholar]
- 6. ) Boonstra , J . Visualization of epidermal growth factor receptor in cryosections of cultures A431 cells by immuno‐gold labeling . Ear. J. Cell Biol , 36 , 209 – 216 ( 1985. ). [PubMed] [Google Scholar]
- 7. ) Mullin , J. M. and Mcginn , M. T.Epidermal growth factor‐induced mitogenesis in kidney epithelial cells (LLC‐PK1) . Cancer Res. , 48 , 4886 – 4891 ( 1988. ). [PubMed] [Google Scholar]
- 8. ) Scheving , L. A. , Shiurba , R. A. , Nguyen , T. D , and Gray , G. M.Epidermal growth factor receptor of the intestinal enterocyte . J, Biol. Chem. , 264 , 1735 – 1741 ( 1989. ). [PubMed] [Google Scholar]
- 9. ) Ozawa , S. , Ueda , M. , Ando , N. , Abe , O. , Minoshima , S. and Shimizu , N.Selective killing of squamous carcinoma cells by an immunotoxin that recognizes the EGF receptor . Int. J. Cancer , 43 , 152 – 157 ( 1989. ). [DOI] [PubMed] [Google Scholar]
- 10. ) Behzadian , M. A. and Shimizu , N.Monoclonal antibody that immunoreacts with a subclass of human receptors for epidermal growth factor . Cell Struct. Funct. , 10 , 219 – 232 ( 1985. ). [DOI] [PubMed] [Google Scholar]
- 11. ) Takeichi , M.The cadherins: cell‐cell adhesion molecules controlling animal morphogenesis Development , 102 , 639 – 655 ( 1988. ). [DOI] [PubMed] [Google Scholar]
- 12. ) White , J. G. , Amos , W. B. and Fordham , M.An evaluation of confocal versus conventional imaging of biological structures by fluorescence light microscopy . J. Cell Biol. , 105 , 41 – 48 ( 1987. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
- 13. ) Gingerich , R. L. , Gilbert , W. R. , Comens , P. G. and Gavin , J. R. . III. Identification and characterization of insulin receptors in basolateral membranes of dog intestinal mucosa . Diabetes , 36 , 1124 – 1129 ( 1987. ). [DOI] [PubMed] [Google Scholar]
- 14. ) Anklesaria , P. , Teixido , J. , Laiho , M. , Pierce , J.H. , Greenberger , J. S. and Massague , J.Cell‐cell adhesion mediated by binding of membrane‐anchored transforming growth factor alpha to epidermal growth factor receptors promotes cell proliferation . Proc. Natl. Acad. Sci. USA , 87 , 3289 – 3293 ( 1990. ). [DOI] [PMC free article] [PubMed] [Google Scholar]