Physico-Chemical Properties, Aerosolization and Dissolution of Co-Spray Dried Azithromycin Particles with L-Leucine for Inhalation

Abstract

Purpose

Inhalation therapy is popular to treat lower respiratory tract infections. Azithromycin is effective against some bacteria that cause respiratory tract infections; but it has poor water solubility that may limit its efficacy when administrated as inhalation therapy. In this study, dry powder inhaler formulations were developed by co-spray drying azithromycin with L-leucine with a purpose to improve dissolution.

Methods

The produced powder formulations were characterized regarding particle size, morphology, surface composition and in-vitro aerosolization performance. Effects of L-leucine on the solubility and in-vitro dissolution of azithromycin were also evaluated.

Results

The spray dried azithromycin alone formulation exhibited a satisfactory aerosol performance with a fine particle fraction (FPF) of 62.5 ± 4.1%. Addition of L-leucine in the formulation resulted in no significant change in particle morphology and FPF, which can be attributed to enrichment of azithromycin on the surfaces of composite particles. Importantly, compared with the spray-dried amorphous azithromycin alone powder, the co-spray dried powder formulations of azithromycin and L-leucine demonstrated a substantially enhanced in-vitro dissolution rate. Such enhanced dissolution of azithromycin could be attributed to the formation of composite system and the acidic microenvironment around azithromycin molecules created by the dissolution of acidic L-leucine in the co-spray dried powder. Fourier transform infrared spectroscopic data showed intermolecular interactions between azithromycin and L-leucine in the co-spray dried formulations.

Conclusions

We developed the dry powder formulations with satisfactory aerosol performance and enhanced dissolution for a poorly water soluble weak base, azithromycin, by co-spray drying with an amino acid, L-leucine.

INTRODUCTION

Azithromycin is a broad-spectrum antibiotic with substantial antibacterial activities against Gram-positive and Gram-negative pathogens (1). It has been widely used to treat lower respiratory tract infections (1–3). It also exhibits anti-inflammatory (4), muco-regulatory (5), and anti-biofilm (6) activities. Azithromycin has been approved by the FDA for treatment of community acquired pneumonia and exacerbations of chronic obstructive pulmonary disease (COPD). Furthermore, it is effective against other respiratory tract disorders, like bronchiectasis, nontuberculous mycobacterial pulmonary diseases, and pulmonary nocardia infection (7–10). However, long-term use of systemically administrated azithromycin therapies may lead to adverse effects including gastrointestinal symptoms (11) and hearing loss (12). Local delivery of azithromycin for lower respiratory tract infections may minimize such systemic adverse events while maintain the treatment efficacy (13).

Inhaled formulations are attractive for treatment of respiratory diseases (14). The treatment of respiratory diseases by delivering the therapeutics to the site of diseases offers rapid onset of drug action, high therapeutic efficacy and reduced systemic exposure (15). In-vivo animal and clinical studies have shown superior efficacy and safety profiles of some inhaled antibiotic therapies over traditional systemic routes of drug delivery (16). Among all inhaled devices for antibiotic delivery, dry powder inhalers (DPIs) are becoming a popular form because in general they are easy to use, portable and most of APIs are chemically more stable than in powder form than the liquid counterparts (17).

In pulmonary drug delivery systems, aerosolized fine drug particles (typically aerodynamic diameter 1–5 μm) can be efficiently delivered to the target sites in the lower airways (18). However, such fine powders produced by the traditional jet milling process generally have poor flow and poor aerosolization properties (17,19), which are the main challenges for formulation and manufacturing (20). These are because fine particles tend to stick to each other, forming agglomerates due to the strong inter-particle cohesive forces (21). In practice, fine drug particles are mixed with coarse carriers to improve the flow and ensure the consistency of aerosol performance (22,23). However, such carrier-based DPI formulations may not be suitable for high dose antibiotics because the drug load of a single dose is substantially limited by the inclusion of carriers (24). Particle engineering is of growing interest as an effective approach to improve the flow and aerosolization of the drug particles by reducing intrinsic powder cohesion (25–27). Spray drying is one of the most popular approaches to engineer the inhalable powder formulation to improve aerosol performance (28,29).

Several studies have attempted to develop inhalable powder formulation of azithromycin. Young et al., co-spray dried azithromycin with mannitol (a mucolytic agent) for the treatment of bronchiectasis. The co-sprayed formulation demonstrated a fine particle fraction (≤6.8 μm; over emitted dose) of 82% ± 2.1% through an Orbital DPI device (30). Li et al., reported that higher glass transition temperature (Tg) correlated with a higher fine particle fraction and lower MMAD, in an azithromycin-mannitol co-spray dried formulation (31). Li et al., also reported that spray feed pump rate could significantly affect the aerosol properties of spray dried azithromycin powders (32). An in-vivo study in rats demonstrated that intratracheal administration of azithromycin dry powder achieved significantly higher drug concentrations in lung epithelial lining fluid (ELF) as compared with the intravenous route (33).

The mechanism of antimicrobial activity for azithromycin is that azithromycin binds to the 50S ribosomal subunit of susceptible microorganisms and interferes with bacterial protein synthesis (34). To be effective against bacteria on the airway surfaces, the deposited azithromycin particles should dissolve, which then generate relatively high drug concentrations at the infection sites and drug can enter the bacterial cells. Undissolved drug particles may not be able to enter bacterial cells and be cleared by macrophage uptake or mucociliary clearance (35). Unfortunately azithromycin is poorly water soluble (1,3), and such low water solubility and poor dissolution properties may compromise the efficacy of inhaled azithromycin therapies.

Formulating poorly water soluble drugs into amorphous forms is a recognized approach to improve solubility, dissolution and efficacy (36,37). Amino acids have been investigated for improving the dissolution of poorly soluble drugs by forming co-amorphous dispersions (38–42). It is noted that the weak basic azithromycin exhibits a pH dependent solubility profile (43). Given amino acids’ weakly acidic nature, we hypothesized that formulating azithromycin with L-leucine may improve the solubility and dissolution of azithromycin by providing a more acidic environment. L-leucine has been extensively investigated in DPI formulations to reduce cohesion and improve aerosol performance (44–46) and moisture protection (47); however, its capability to improve dissolution of inhalable poorly water soluble DPI formulations has not been systemically examined. In the present study, we developed co-spray dried inhalable powder formulations consisting of azithromycin and L-leucine. The formulations were characterized to investigate the effects of L-leucine on the physicochemical properties as well as aerosolization, solubility and dissolution of azithromycin. The chemical structures of azithromycin and L-leucine are provided in Appendix 1.

MATERIALS AND METHODS

Materials

Azithromycin dihydrate was purchased from ßetaPharma^® (Shanghai) Co., Ltd. (Wujiang City, Jiangsu Province, China). L-leucine was supplied by Sigma–Aldrich (St. Louis, Missouri, USA). Methanol (HPLC grade) was supplied from Merck (Fair Lawn, New Jersey, USA).

Spray Drying

A Büchi 290 spray dryer (Büchi Labortechnik AG, Falwil, Switzerland) was employed to produce the powder formulations. Briefly, formulation components (L-leucine alone, azithromycin alone, combinations with molar ratios of 1:1 and 4:1) were dissolved in the co-solvent of water: ethanol (1:1, v/v). Each solution was spray-dried at the same parameters: inlet temperature 80 ± 2°C; outlet temperature 48 ± 2°C; aspirator 35 m³/h; atomizer setting 700 L/h; feed rate 2 mL/min. Total solid concentration of the feed solutions was kept constant (10 mg/mL) for different formulations. The samples were kept in a desiccator with silica gel at 20 ± 3°C.

Scanning Electron Microscopy (SEM)

Morphology of the spray-dried particles was examined by a field emission scanning electron microscope (NOVA nanoSEM, FEI Company, Hillsboro, Oregon, USA). Adhesive carbon tape was placed on stainless steel stub and then a small amount of the sample was spread over the tape. Excessive powder was removed by pressurized air. The stubs were then coated with platinum at 40 mA for 1 min using a sputter coater (208 HR, Cressington Sputter Coater, England, UK), corresponding to approximately 10–30 nm coating thickness. The SEM images were captured using an inbuilt software.

Physical Particle Size

The scanning electron microscopy images captured at a fixed magnification were used to determine particle size of the samples (48). Martin’s diameter of approximately 150 randomly-selected particles from three different images was measured for each sample using the ImageJ software (National Institute of Health, Rockville, Maryland, USA). D₁₀ (diameter at 10% undersize), D₅₀ (diameter at 50% undersize), and D₉₀ (diameter at 90% undersize) were calculated according to standard procedures (49).

Powder X-ray Diffraction (PXRD)

Crystallinity was determined by powder X-ray diffraction (Rigaku Smartlab^™ diffractometer, Rigaku Americas, Texas, USA). A Cu-Kα radiation source and a D/tex ultra-detector were used. The samples were spread on a glass slide and placed in the measuring chamber. The PXRD scanning was set as 5 to 40° 2θ at 5°/min with a voltage of 40 kV and a current of 44 mA.

X-ray Photoelectron Spectroscopy (XPS)

Surface composition of the composite formulations was evaluated quantitatively by X-ray photoelectron spectroscopy (XPS) (AXIS Ultra DLD spectrometer, Kratos Analytical Inc., Manchester, UK) with monochromic Al Kα radiation (1486.6 eV) using pass energy (PE) of 20 and 160 eV for high-resolution and survey spectra, respectively. A commercial Kratos charge neutralizer was used to avoid non-homogeneous electric charge of non-conducting powder and to achieve better resolution. Typical instrument resolution for PE of 20 eV is ~0.35 eV. Binding energy (BE) values refer to the Fermi edge and the energy scale was calibrated using Au 4f7_/2 at 84.0 eV and Cu 2p_3/2 at 932.67 eV. Powder samples were placed on a stainless-steel sample holder bar using a double-sided sticking Cu tape. A CasaXPS software (version 2313 Dev64) was applied to process the XPS data. Curve-fitting was performed following a Shirley background subtraction using model peaks obtained from pure compounds. Atomic concentrations of the elements in the near-surface region were estimated after a Shirley background subtraction considering the corresponding Scofield atomic sensitivity factors and inelastic mean free path (IMFP) of photoelectrons using standard procedures in the CasaXPS software assuming homogeneous mixture of the elements within the information depths (~10 nm). The % surface composition was calculated using the C 1 s curve-fitting (50).

Time-of-Flight Secondary Ion Mass Spectrometry (ToF-SIMS)

Surface composition of the composite formulations was characterized using Time-of-flight secondary ion mass spectrometry (nanoToF instrument, Physical Electronics Inc., Chanhassen, Minnesota, USA) as described elsewhere with slight modifications (51). Data were obtained from 4 areas (75 × 75 μm each) per sample. Characteristic peak fragments for azithromycin and L-leucine were identified. For azithromycin, the peaks at m/z ~98 atomic mass unit (amu) and ~158 amu were selected corresponding to [C₆H₁₂N⁺] and [C₈H₁₆NO₂⁺] fragments, respectively. For L-leucine, the fragment at m/z ~ 132 amu corresponding to [C₆H₁₄NO₂⁺] was selected as the characteristic peak. A WincadenceN software (Physical Electronics Inc., Chanhassen, Minnesota, USA) was employed to construct high-resolution surface composition overlays.

Solid State Fourier Transform Infrared Spectroscopy (FTIR) Spectroscopy

The raw materials and selected spray-dried formulations were analyzed using a Cary 600 series IR spectrophotometer (Agilent Technologies, Santa Clara, California, USA) equipped with an attenuated total reflectance (ATR) sample stage. Samples were analysed at a resolution of 4 cm⁻¹ in the range on 400–4000 cm⁻¹. A background scan was collected prior to collecting the sample spectra to minimize the interferences of water and CO₂ signals (52).

Dynamic Vapor Sorption

Dynamic vapor sorption (DVS-Intrinsic, Surface Measurement Systems Ltd., London, UK) was employed to measure moisture sorption behavior. Each sample was exposed to 0% relative humidity (RH) at the beginning of measurement to provide a baseline. The sorption cycle was set from 0 to 90% RH, and 90 to 0% for the desorption cycle. Equilibrium mass change at each testing RH was determined.

Drug Quantification

Concentration of azithromycin was measured by a validated high performance liquid chromatography (HPLC) method (53). Briefly, the HPLC system consisted of 1260 Quat Pump, 1290 Thermostate, 1260 ALS autosampler, 1260 TCC thermostatic column compartment, 1260 VWD variable wavelength detector and an Eclipse Plus column (C18, 150 × 4.60 mm, 5 μm) (all were supplied by Agilent, Waldbronn, Germany). The mobile phase was composed of: (A) 20 mM potassium dihydrogen phosphate (pH was adjusted to 7 with 10% w/v sodium hydroxide) and methanol (B). The isocratic elution program used for azithromycin detection was 80% A and 20% B v/v for 15 min at the flow rate of 1.0 mL/min at a wavelength of 210 nm. The retention time for azithromycin was 12 mins. A calibration curve of azithromycin was linear (r² > 0.999) in the required concentration range (0.0125–0.5 mg/mL).

In-Vitro Aerosol Performance

A Multi-Stage Liquid Impinger (MSLI) (Copley Scientific Limited, Nottingham, UK) was used to evaluate In-vitro aerosol performance with a USP induction port (USP throat). Each capsule (size 3 hydroxypropyl methylcellulose capsules, Qualicaps, Whitsett, North Carolina, USA) was loaded with 10 ± 2 mg of powder and dispersed through an RS01 DPI device (Plastiape S.p.A., Osnago, Italy). Aerosol performance was tested using a standard dispersion procedure: 4 L of air was drawn to pass through the inhaler at an airflow of 100 L/min for 2.4 s, with a pressure drop of approximately 4 kPa at 100 L/min across the device (54). The cutoff diameters were 10.4, 4.9, 2.4, and 1.2 μm for Stages 1–4 of the MSLI, respectively. Drug retained in capsule, device, USP throat, Stages 1–4 and filter paper were dissolved with 20 mL of a co-solvent (20 mM potassium dihydrogen phosphate adjusted to pH 5: methanol = 1:1 v/v). Emitted dose (ED) was defined as the collected drug except for those retained in the capsule and device, over the total recovered drug. Fine particle fraction (FPF) represents the fraction of the drugs deposited on Stage 3, Stage 4 and filter paper over the recovered dose.

Equilibrium Solubility

The equilibrium azithromycin solubility was determined. Briefly, an excess of azithromycin (i.e., > 5 mg/mL equivalent of azithromycin of each formulation) was added to 5 ml of dissolution media (phosphate buffer saline, PBS (pH 7.4), potassium chloride buffer (pH 2) and carbonate buffer (pH 11) maintained at 37 ± 1°C. Buffer media were used instead of water to reflect potential buffering effects of lung lining fluid. The resultant suspensions were constantly stirred at 500 rpm (VWR International, Arlington Heights, Illinois, USA) with a magnetic bar (12 mm, VWR, Arlington Heights, Illinois, USA). After 24 h the suspensions were filtered using a 0.45 μm nylon syringe filter (VWR, Arlington Heights, Illinois, USA) and the concentrations of azithromycin was determined by HPLC.

In-Vitro Dissolution

Dissolution kinetics of the formulations were evaluated using two methods: beaker method and Franz diffusion cell method using the procedures described previously with minor modifications (55). Briefly, dissolution studies were carried out with aerosolized particles collected on the Stage 4 of the next generation impactor (NGI, Copley Scientific Limited, Nottingham, UK) (55). Two capsules (size 3 HPMC capsules, Qualicaps, Whitsett, North Carolina, USA), with each containing 10 ± 2 mg of the powder formulation, were actuated. For each dispersion, standard dispersion procedure were used: 4 L of air was allowed to pass the inhaler at 100 L/min for 2.4 s (55). The powder in the stage 4 of NGI was collected using a filter disk (Whatman® Grade 2, pore size 5 μm, GE Healthcare, Parramatta, Australia) for dissolution studies. The dissolution media was 20 mL of PBS (pH 7.4) to reflect potential buffering effects of lung lining fluid. An aliquot of 0.2 mL sample was drawn at time intervals of 2, 5, 10, 15, 20, 30, 45, 60, 90, 120, 150 and 180 mins, with a compensation of 0.2 mL fresh medium immediately after sample collection.

In the beaker method, the filter disk was gently placed in 20 mL PBS (pH 7.4 at 37 ± 2°C) held in a 100-mL jacketed beaker (4.7 mm internal diameter × 8.5 cm height, Vineland, New Jersey, USA). The dissolution media was constantly stirred at 500 rpm with the help of magnetic stirrer (VWR, Arlington Heights, Illinois, USA) using a polygon magnetic bar (12 mm, VWR, Arlington Heights, Illinois, USA).

In the Franz cell diffusion method, Franz cell (V6B, PermeGear Inc., Hellertown, Pennsylvania, USA) reservoirs were filled with 20 mL PBS (pH 7.4) and maintained at 37 ± 1°C. The dissolution media was stirred constantly at 600 rpm (6-station Franz Cell stirrer, PermeGear Inc., Hellertown, Pennsylvania, USA) with a magnetic bar (12.5 mm). The stirring speed is fixed at 600 rpm by the manufacturer, which is not adjustable. The filter disk with the aerosolized powder was placed on the top of the Franz cell, being in contact with the dissolution media (55).

Statistical Analysis

The statistical analysis was conducted using one-way analysis of variance (ANOVA) with Tukey–Kramer post-hoc test or independent t-test using a GraphPad Prism software (GraphPad Software, Inc., La Jolla, California, USA).

RESULTS

Scanning Electron Microscopy (SEM)

SEM images of the spray-dried formulations are presented in Fig. 1. The Azithromycin-SD particles had a near-spherical shape and corrugated surfaces (Fig. 1a). Composite formulations showed the similar morphology to the Azithromycin-SD particles (Fig. 1b and c) rather than the spray dried L-leucine (47). Previous studies have shown that L-leucine could alter the morphology of some spray-dried particles due to enrichment of L-leucine on the particle surface (56). Our SEM data showed that L-leucine had no apparent effects on the morphology of spray-dried azithromycin particles. Thus, we proposed that L-leucine was not dominant on the surface in these spray-dried composite formulations, which was confirmed by below surface chemical composition analysis.

Fig. 1.

Representative scanning electron microscopy images of: Azithromycin-SD (a1 and a2); L-leucine-Azithromycin_1–1 (b1 and b2); L-leucine-Azithromycin_4–1 (c1 and c2).

Physical Particle Size

Particle sizes of the formulations are presented in Table I. The D₅₀ values of all spray-dried formulations were less than 2 μm. The L-leucine-Azithromycin_4–1 had slightly larger sizes than the Azithromycin-SD and L-leucine-Azithromycin_1–1. The D₉₀ values of all the formulations were less than 3 μm, indicating the fine particle sizes for all formulations.

Table I.

Particle Sizes of the Spray-Dried Powder Formulations

Formulation	Particle size (μm)
	D10	D50	D90
Azithromycin-SD	0.6 ± 0.1	0.9 ± 0.1	1.4 ± 0.2
L-leucine-Azithromycin_1–1	0.9 ± 0.1	1.2 ± 0.2	1.3 ± 0.0
L-leucine-Azithromycin_4–1	1.4 ± 0.2	1.9 ± 0.3	2.1 ± 0.2

X-ray Photoelectron Spectroscopy (XPS)

The % theoretical composition of L-leucine and azithromycin was calculated by normalizing the molar composition with the number of carbons from each molecule, which gives the contribution of % carbon atom from each molecule. These data can be directly compared with the % surface composition obtained via C 1 s curve-fits from XPS, which represents the surface fraction of carbon atom from L-leucine and azithromycin. In Table II, the XPS data demonstrated that azithromycin concentration on the particle surface was significantly higher than the theoretical ratio, indicating surface enrichment of azithromycin. For example, as for L-leucine-Azithromycin_4–1, the theoretical concentration of azithromycin was 61.3%; while the measured surface concentration was 90.8% (as determined by C1s curvefits).

Table II.

Theoretical and Measured (by XPS) Surface Compositions of the Spray-Dried Formulations

Formulation	% Theoretical composition		% Surface Composition
	L-leucine	Azithromycin	L-leucine	Azithromycin
L-leucine-Azithromycin _1–1	13.6	86.4	2.4	97.6
L-leucine-Azithromycin_4–1	38.7	61.3	9.2	90.8

Time-of-Flight Secondary Ion Mass Spectrometry (ToF-SIMS)

In the ToF-SIMS images (Fig. 2), the green signal represents azithromycin, and the red signal represents L-leucine. For the L-leucine-Azithromycin_1–1, the azithromycin signals are overwhelming on the surface and significantly more enriched on the surface than the L-leucine-Azithromycin_4–1 particles. Such observations are in agreement with the XPS data.

Fig. 2.

Distributions of L-leucine (red) and azithromycin (green) on the particle surfaces measured by ToF-SIMS: (a): Azithromycin-SD; (b): L-leucine-Azithromycin _1–1; (c): L-leucine-Azithromycin_4–1; and (d): L-leucine (scale bar represents 10 μm).

Solid State Fourier Transform Infrared Spectroscopy (FTIR)

FTIR was employed to investigate the potential interactions between L-leucine and azithromycin at the molecular level in the co-spray dried formulations (57). First, IR spectra of the Azithromycin-SD and the raw azithromycin (dihydrate) were compared (Fig. 3). In the spectrum of raw azithromycin dihydrate, sharp peaks at 3492 and 3559 cm⁻¹ (gray area) were clearly observed. These distinct peaks can be assigned to the O–H stretching modes attribute to the presence of ‘tightly bound’ water in the crystal lattice (58). Interestingly, these peaks disappeared in the Azithromycin-SD indicating conversion of azithromycin dihydrate to an anhydrous form (58). The amorphous nature of this anhydrous form of azithromycin was further confirmed by the PXRD data in the following section. Because azithromycin maintained its amorphous form in the co-spray dried formulation with L-leucine, the IR spectrum of the Azithromycin-SD was used as the reference in the following context.

Fig. 3.

FTIR spectra of the raw azithromycin (dihydrate) and Azithromycin-SD (amorphous).

In Fig. 4, we observed that azithromycin is largely infrared inactive in the 1500–1700 cm⁻¹ spectral region, while L-leucine is infrared active. To be specific, the L-leucine-SD demonstrates two distinct peaks at 1513 and 1579 cm⁻¹, which can be assigned to the carbonyl stretching on the amino acid. It has been reported that these two carbonyl peaks in a lower wavenumber were attributed to the formation of intramolecular hydrogen bonding between NH₂ moiety and carbonyl oxygen (59). Hence, breaking such intramolecular interactions can lead to the carbonyl peaks shifting towards higher wavenumber. Interestingly, hypsochromic shifts of Peak A (from 1513 to 1526 cm⁻¹) and Peak B (from 1579 to 1584 cm⁻¹) in the IR spectra of the co-spray dried formulation are noted (52,60). These blue shifts indicate the new molecular interactions are formed during the process of co-spray drying azithromycin with L-leucine. By breaking the intramolecular interactions discussed above, carboxylic acid group can work as a strong hydrogen bond donor to interact with the hydrogen bond accepting group of L-leucine. There are many possible hydrogen bond acceptors located at the azithromycin to potentially form intermolecular interactions with L-leucine. However, it is very challenging to confirm the interaction center on azithromycin due to its complex chemical structure and the limited spectral information. Further investigation of the molecular interactions in the azithromycin-L-leucine complex will be warranted.

Fig. 4.

FTIR spectra of the spray-dried formulations.

Dynamic Water Vapor Sorption (DVS)

DVS data showed that L-leucine-SD absorbed negligible water (<1% w/w) even at very high relative humidity (Fig. 5). Azithromycin-SD absorbed relatively higher amounts of water (~5% w/w), which was reversible during desorption. This indicates that water molecule was not associated chemically with azithromycin and no crystallization occurred during the measurement. L-leucine-Azithromycin_1–1 showed similar moisture sorption and desorption profiles as the Azithromycin-SD. L-leucine-Azithromycin_4–1 demonstrated less water sorption than Azithromycin-SD, but higher than L-leucine-SD. This reduction in hygroscopicity of L-leucine-Azithromycin_4–1 as compared with Azithromycin-SD is attributed to higher concentration of non-hygroscopic L-leucine.

Fig. 5.

Dynamic water sorption behavior of the spray-dried formulations.

Powder X-ray Diffraction (PXRD)

PXRD diffractograms of the raw azithromycin and raw L-leucine showed sharp peaks indicating they were crystalline (Fig. 6a). L-leucine-SD showed certain degrees of crystallinity that reflect their fast crystallization behavior right after spray drying. In contrast, Azithromycin-SD did not exhibit any crystalline peaks, indicating it was amorphous (Fig. 6b). Interestingly, the L-leucine crystalline peaks were also evident in the diffractograms of composite formulations with a broader shape and the intensity of these peaks decreased with an increase in azithromycin proportion (Fig. 6b). The physical stability of the spray-dried formulations stored at 55% RH and 75% RH was also tested using PXRD (Fig. 6c and d). There was no significant changes in crystalline peaks indicating that spray-dried formulations were physically stable upon short-term exposure to the elevated humidity.

Fig. 6.

Power X-ray diffraction patterns of: (a) raw azithromycin and L-leucine; (b) freshly spray-dried formulations; (c) spray-dried formulations stored at 55% RH for a week; (d) spray-dried formulations stored at 75% RH for a week.

In-Vitro Aerosol Performance

All formulations demonstrated similar ED around 80% and FPF around 62%. The Azithromycin-SD showed a fine particle fraction of 62.5 ± 4.1% (Fig. 7a). Composite formulations of L-leucine-Azithromycin_1–1 and L-leucine-Azithromycin_4–1 showed fine particle fractions of 62.9 ± 2.3% and 62.3 ± 2.2%, respectively, (Fig. 7a) suggesting that L-leucine had no effect on the aerosolization of azithromycin. Previous studies have shown that L-leucine can act as the force control agent to reduce the cohesion and improve the aerosolization upon co-spraying (61,62). The capability of L-leucine to reduce cohesion is believed to be associated with its enrichment on the particle surface, which allows it to produce rougher particles with lower surface energy (56). Our results showed that L-leucine did not enrich on the surfaces of the composite formulations and have no significant impact in aerosolization. This suggests that surface enrichment of L-leucine is pivotal to aerosolization enhancing capability. We also investigated the effect of elevated humidity on the aerosolization of the spray-dried formulations. The results indicated short-term exposure to elevated humidity caused no significant change in the ED and FPF (Fig. 7b). A detailed deposition profiles of different formulations is provided in Appendix 2. The aerodynamic size seems larger for the spray dried formulations with higher L-leucine concentration than the Azithromycin-SD. For instance, there was an increase in azithromycin deposition in Stage 3 and a decrease in Filter when the L-leucine concentration was increased. This is in agreement with the marginal increases in physical size of the spray dried formulations with L-leucine (Table I). However, such minor difference in aerodynamic size distribution should not contribute to different dissolution behavior because the particles used for dissolution were collected from the same aerodynamic size fraction in Stage 4 of NGI.

Fig. 7.

Fine particle fraction (FPF) (%) and emitted dose (ED) (%) of the spray-dried formulations stored at: (a) 55% RH; and (b) 75% RH. The data are presented as mean ± SD (n = 4). NS denotes not significant (p > 0.05).

Equilibrium Solubility

The solubility of raw azithromycin was 1.0 mg/mL (Table III). The Azithromycin-SD demonstrated a similar solubility (1.0 mg/mL) compared with the raw azithromycin (p > 0.05). It is not surprising that Azithromycin-SD has a similar equilibrium solubility to the raw crystalline drug as the crystallization likely occurs during the 24-h measurement. The formulations spray-dried with L-leucine showed greater solubility than the Azithromycin-SD (p < 0.05). A trend was observed that the solubility increased with increasing L-leucine concentration. L-leucine-Azithromycin_1–1_Physical Mixture has a similar solubility to the raw azithromycin and L-leucine-Azithromycin_4–1_Physical Mixture only showed a minor increase. Solubility of the spray-dried composite formulations was higher than the corresponding physical mixtures (p < 0.05).

Table III.

Equilibrium Solubility of Azithromycin in Various Formulations (Mean ± SD, n = 4)

Formulation	Azithromycin solubility (mg/mL) at 37°C
Azithromycin-Raw	1.0 ± 0.1
L-leucine-Azithromycin_1–1_Physical Mixture	1.1 ± 0.1
L-leucine-Azithromycin_4–1_Physical Mixture	1.3 ± 0.1
Azithromycin-SD	1.0 ± 0.1
L-leucine-Azithromycin_1–1	1.3 ± 0.1
L-leucine-Azithromycin_4–1	1.8 ± 0.2

In-Vitro Dissolution

Beaker Method

The results measured using the beaker method indicated that the composite formulations had faster dissolution rate compared with the Azithromycin-SD (Fig. 8a). As for the Azithromycin-SD, only 62.0 ± 5.0% of azithromycin was dissolved in 30 min and the maximum drug release obtained was >90% after 180 min. Addition of L-leucine in the formulation was shown to increase the release of azithromycin with >75% and >85% of drug released within 30 min for L-leucine-Azithromycin_1–1 and L-leucine-Azithromycin_4–1, respectively. More than 90% of the drug was released within 60 min for the composite formulations.

Fig. 8.

In-vitro dissolution of azithromycin from the spray-dried formulations as determined by: (a) beaker method; and (b) Franz cell method. Data are presented as mean ± SD (n = 4).

Franz Cell Method

The Franz cell dissolution data demonstrated that for the Azithromycin-SD, only 55.5 ± 4.3% of the drug was dissolved after 120 min (Fig. 8b). However, the azithromycin released after 90 min was >60% for the L-leucine-Azithromycin_1–1 and >80% for the L-leucine-Azithromycin_4–1. At 180 min, the total drug released was 66.2 ± 4.3% for the Azithromycin-SD, whereas >85% of azithromycin was released for both composite formulations. The results indicate that addition of L-leucine in the spray drying formulations substantially improved the dissolution rate of azithromycin, which are in agreement with the beaker method data.

DISCUSSION

Drug concentration in the ELF is vital to the effective treatment of lower respiratory tract infections (16). However, for many antibiotics oral or parenteral administrations only result in low and suboptimal drug concentrations on the airway surfaces (16). Inhalation therapy enables direct delivery of drugs to the infection sites on the lower airway surfaces. Recent studies have attempted to develop inhaled azithromycin formulations (13,30–33).

For dry powder formulations, dissolution of the drug aerosol particles deposited on the airway surfaces is critical to the antimicrobial efficacy. For some drugs with high water solubility and fast systemic absorption such as ciprofloxacin HCl, there may be a need to develop controlled released formulations to achieve high local drug concentrations for longer time (63). However, for poorly water soluble drugs, there is a need to improve the dissolution and maintain drug concentrations in the lung lining fluid above minimum inhibitory concentrations (16,64,65), particularly for azithromycin which has to enter the bacterial cells to be effective (34). Given lung surface is not an ideal place for drug dissolution due to the low fluid volume and particle clearance mechanisms (66), dissolution behavior is crucial for the efficacy of inhaled azithromycin DPIs. However, there is a scarce in the literature that examines the dissolution behavior of inhalation formulations of azithromycin, a poorly water-soluble drug. Thus, the main objective of this study was to develop composite dry powder inhaler formulations of azithromycin with L-leucine, and characterize the formulations regarding aerosolization and dissolution.

L-leucine is commonly employed as a force control agent to improve the aerosolization of spray dried fine powders (61,62). It is believed that the addition of L-leucine increases the surface roughness and reduces surface energy of the spray-dried particles as consequences of L-leucine enrichment on the particle surface (56,67–69). However, in this study, co-spray drying with L-leucine had no significant effects on the FPF of azithromycin. Our results indicated that co-spray drying with L-leucine resulted no apparent change in the morphology compared with the Azithromycin-SD particles. This suggests that surface enrichment of L-leucine is essential to alter particle morphology and improve the aerosolization of co-sprayed dried powder formulations, which is associated with the self-assembly behavior of L-leucine on the surfaces of spray-dried formulations (67,68). Interestingly, our results indicated that surfaces concentration of L-leucine was lower than the theoretical value. This can be attributed to rapid evaporation of ethanol or low solubility of azithromycin in the co-solvent system, resulting in super-saturation of azithromycin in aqueous phase of the droplet. Because of low diffusion rate of azithromycin towards to the core of the droplet and rapid precipitation in the drying phase, particle surfaces are enriched with azithromycin (53,55,70). All the spray-dried formulations exhibited satisfactory physical stability even upon short-term storage at the elevated humidity, which was attributed to the relative hydrophobic nature of azithromycin as indicated by the DVS data.

Spray-drying with L-leucine was shown to accelerate the dissolution of azithromycin as measured by two different testing methods, in comparison to the Azithromycin-SD powder. We would highlight that while there was an increase in the particle size with increasing L-leucine feed concentration, such size differences are unlikely to affect the dissolution kinetics of the resultant formulations. This is because the dissolution studies were carried out with the particles in the aerodynamically classified fraction i.e., the particles deposited in Stage 4 of the NGI. Thus, any change in the dissolution kinetics are not due to the change in particle size.

The solubility (Table III) for the L-leucine-containing formulations is much higher than the amorphous azithromycin only formulation, and there is an increase in solubility when the L-leucine concentration increases. These data have shown that improvements in solubility and dissolution are not only the consequence of the amorphous form. We have also measured the equilibrium solubility of azithromycin with presence of mannitol (31), but no change was observed (1 mg/mL). A few factors can contribute to the enhanced dissolution. First, azithromycin and L-leucine likely formed a complex as supported by intermolecular interactions between the two components in the composite formulation (60,71). To be specific, the peaks assigned to the carboxylic acid group of L-leucine were hypsochromically shifted suggesting that the formation of hydrogen bond with azithromycin changed the stretching mode of carbonyl group (Fig. 4). Moreover, L-leucine has much higher aqueous solubility than azithromycin. In the composite formulations, each particle contains both azithromycin and L-leucine as evident by the ToF-SIMS data. Mixing of azithromycin with water-soluble L-leucine at the sub-micron or even molecular level could enhance the dissolution behavior of poorly water-soluble azithromycin (72).

We conducted solubility experiments for Azithromycin-SD and composite formulations in dissolution media with acidic (potassium chloride buffer, pH 2), neutral (PBS, pH 7.4) and basic pH (carbonate buffer, pH 11). Solubility of azithromycin reduced with the increased pH; addition of L-leucine was shown to increase the solubility of azithromycin (Fig. 9). Due to its weakly basic nature, azithromycin tends to increase the pH of the media upon dissolving in potassium chloride buffer and PBS at the end of solubility experiments (24 h); the changes in pH at pH 7.4 are marginal and unlikely have safety concerns considering the buffering capacity of airway lining fluids (73). In the media of pH 11, the dissolving of L-leucine-containing formulations decreased the media pH. These data indicate a minor change in environmental pH can alter the solubility of azithromycin substantially (33). Thus, it is likely that due to the acidic nature of L-leucine, L-leucine decreases the pH of the dissolution media and creates an acidic microenvironment surrounding the azithromycin, although majority of the surfaces are azithromycin. Further investigations are warranted to fully understand the mechanisms of such dissolution enhancement by co-spray drying.

Fig. 9.

Azithromycin solubility at 37°C in: (a) carbonate buffer at pH 11, (b) PBS at pH 7.4 and (c) potassium chloride buffer (pH 2): (□) L-leucine-Azithromycin_4–1; (⋄) L-leucine-Azithromycin_1–1; (○) Azithromycin-SD. Data are presented as mean ± SD (n = 4).

CONCLUSIONS

Traditionally, L-leucine has been widely used to reduce the cohesion and improve the aerosolization of spray dried inhalable powder formulations. Interestingly, in the present study L-leucine did not enrich on the surface of the composite particles containing hydrophobic azithromycin and had no apparent effects on the aerosolization performance. This suggests that improvement of aerosolization by spray drying drugs with L-leucine is dependent on whether L-leucine can be dominant on the particle surface.

The composite formulations exhibited excellent aerosol performance and physical and aerosolization stability against short-term exposure to elevated humidity. Our results showed that co-spray drying with L-leucine improved the dissolution of poorly water-soluble azithromycin. Such enhanced dissolution behavior is attributed to the formation of composite system of azithromycin with water-soluble L-leucine, and the creation of acidic dissolution microenvironment around azithromycin (a weak base) during dissolution. Such increased dissolution of poorly water-soluble azithromycin by co-spray drying with L-leucine may be transferred to the enhanced antimicrobial activities, which deserve further investigations.

Appendix 1

Fig. 10.

Chemical structures of: (a) azithromycin; (b) L-leucine.

Appendix 2

Fig. 11.

Deposition profiles of spray-dried formulations at various stages of MSLI after storage for 1 week at 55% RH (a) and 75% RH (b).