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. 1991 Dec;82(12):1406–1412. doi: 10.1111/j.1349-7006.1991.tb01813.x

Detection of Human Antibody against the Human Papillomavirus Type 16 E7 Protein

Madoka Hashido 1,✉, Tadahito Kanda 2, Soichi Zanma 3, Sumie Watanabe 2, Naoki Komiyama 1,†, Hiroyuki Yoshikawa 4, Nobuo Yamaguchi 1, Takashi Kawana 5, Kunito Kunito 2,1
PMCID: PMC5918352  PMID: 1663920

Abstract

We examined 500 human sera for the presence of antibody against the human papillomavirus type 16 E7 protein by enzyme‐linked immunosorbent assay with bacterially expressed fusion protein lac‐E7, and by radioimmunoprecipitation and immunofluorescence assays with the E7 protein expressed in monkey COS‐1 cells. The anti‐E7 antibody was detectable in 6 out of 54 cases of cervical carcinoma, but in none of the others, including patients with other gynecologic cancers, those with sexually transmitted diseases, and healthy adults. The data indicate that expression of the E7 protein is closely related to cervical carcinoma.

Keywords: HPV 16 E7, Human anti‐E7 antibody, Cervical cancer

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