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Japanese Journal of Cancer Research : Gann logoLink to Japanese Journal of Cancer Research : Gann
. 1991 Dec;82(12):1413–1420. doi: 10.1111/j.1349-7006.1991.tb01814.x

Tissue Distribution of Mouse Mammary Tumor Virus (MMTV) Antigens and Endogenous MMTV Loci in Japanese Laboratory Mouse Strains

Worawidh Wajjwalku 1, Masahide Takahashi 1, Osamu Miyaishi 1, Jin Lu 1, Keita Sakata 1, Toyoharu Yokoi 1, Shinsuke Saga 1, Masao Imai 1, Mutsushi Matsuyama 1,✉, Munemitsu Hoshino 1
PMCID: PMC5918366  PMID: 1663921

Abstract

The distribution of mouse mammary tumor virus (MMTV) antigens was studied by the immunoper‐oxidase method in the II‐TES and I‐TES mouse strains as well as their progenitors, CS and DBA/2 strains. In the II‐TES, I‐TES and CS strains, and BALB/c mice foster‐nursed with these strains, MMTV antigens were found not only in epithelial cells of the mammary glands but also in those of other tissues including the seminal vesicle, vas deferens, epididymis, prostate, parotid, submandibular, lacrimal, sebaceous, and urethral glands. In DBA/2 and BALB/cfDBA/2 mice, however, the MMTV antigens were found only in the mammary glands. Electron microscopic examination showed MMTV particles in these organs. When we examined the presence of Mtv‐1 and 2 proviruses, which are known to be responsible for MMTV expression, in the genomes of the II‐TES, I‐TES, CS and DBA/2 strains by Southern blotting, Mtv‐2 was not found in any of the mice and Mtv‐1 was found in the II‐TES and DBA/2 mice but not in the I‐TES and CS mice. Instead, four new endogenous MMTV loci, which have never previously been reported in laboratory mouse strains, were detected in the genomes of the II‐TES, I‐TES and CS strains. One (designated Mtv‐42) was common in the three strains and the other three (designated Mtv‐43, 44 and 45) were common in the II‐TES and I‐TES strains or the II‐TES and CS strains. These results thus suggest that new endogenous MMTV loci may be responsible for MMTV expression in a variety of tissues of these three strains.

Keywords: Mouse mammary tumor virus, Endogenous Mtv loci, II‐TES strain, I‐TES strain, CS strain

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REFERENCES

  • 1. ) Bentvelzen , P. and Hilgers , J , Murine mammary tumor virus . In “ Virus Oncology ,” ed. Klein G. , pp. 311 – 355 ( 1980. ). Raven Press; , New York . [Google Scholar]
  • 2. ) Moore , D. H. , Long , C. A. , Vaidya , A. B. , Sheffield , J. B. , Dion , A. S. and Lasfargues , E. Y.Mammary tumor viruses . Adv. Cancer Res. , 29 , 347 – 418 ( 1979. ). [DOI] [PubMed] [Google Scholar]
  • 3. ) Nandi , S. and McGrath , C. M.Mammary neoplasia in mice . Adv. Cancer Res. , 17 , 353 – 414 ( 1973. ). [Google Scholar]
  • 4. ) Hynes , N. E. and Groner , B.Mammary tumor formation and hormonal control of mouse mammary tumor virus expression . Curr. Top. Microbiol. Immunol , 101 , 51 – 74 ( 1982. ). [DOI] [PubMed] [Google Scholar]
  • 5. ) Kozak , C. , Peters , G. , Pauley , R. , Morris , V. , Michalides , R. , Dudley , J. , Green , M. , Davisson , M. , Prakash , O. , Vaidya , A. , Hilgers , J. , Verstraeten , A. , Hynes , N. , Diggelmann , H. , Peterson , D. , Cohen , J. C. , Dickson , C. , Sarkar , N. , Nusse , R. , Varmus , H. and Callahan , R.A standardized nomenclature for endogenous mouse mammary tumor viruses . J. Virol , 61 , 1651 – 1654 ( 1987. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 6. ) Morris , V. L. , Medeiros , E. , Ringold , G. M. , Bishop , J. M. and Varmus , H. E.Comparison of mouse mammary tumor virus‐specific DNA inbred, wild and Asian mice and in tumors and normal organs from inbred mice . J. Mol. Biol. , 114 , 73 – 91 ( 1977. ). [DOI] [PubMed] [Google Scholar]
  • 7. ) Dickson , C.Molecular aspects of mouse mammary tumor virus biology . Int. Rev. CytoL. , 108 , 119 – 147 ( 1987. ). [DOI] [PubMed] [Google Scholar]
  • 8. ) Tsubura , A. and Morii , S.Immunohistochemical detection of mammary tumor virus antigens in sweat and sebaceous glands of mice . Exp. Anim. , 34 , 303 – 310 ( 1985. ). [DOI] [PubMed] [Google Scholar]
  • 9. ) Tsubura , A. and Morii , S.Mouse mammary tumor virus (MMTV) particles in extraorbital lacrimal and urethral glands of endogenous MMTV‐carrying mice . Jpn. J. Exp, Med. , 57 , 255 – 260 ( 1987. ). [PubMed] [Google Scholar]
  • 10. ) Bentvelzen , P. and Brinkhof , J.Organ distribution of exogenous murine mammary tumor virus as determined by bioassay . Eur. J. Cancer , 13 , 241 – 245 ( 1977. ). [DOI] [PubMed] [Google Scholar]
  • 11. ) Aoyama , A. , Saga , S. , Nagayoshi , S. , Hoshino , M. , Tomita , T. and Kondo , K.High murine mammary tumor virus expression in milk in a low mammary tumor mouse strain and high incidence of mammary tumor in hybrids produced by crossing with another low mammary tumor mouse strain . Jpn. J. Cancer Res. , 77 , 244 – 249 ( 1986. ). [PubMed] [Google Scholar]
  • 12. ) Aoyama , A. , Nagayoshi , S. , Saga , S. , Malavasi‐Yamashiro , J. , Yokoi , T. , Takenaka , T. , Miyaishi , O. , Lu , J. , Imai , M. , Tomita , T. and Hoshino , M.Genetic resistance to mammary tumorigenesis in a mouse strain with high murine mammary tumor virus expression . Cancer Lett. , 36 , 119 – 123 ( 1987. ). [DOI] [PubMed] [Google Scholar]
  • 13. ) Kondo , K. and Esaki , K.Breeding of tester strains for coat colour genes . Bull. Exp. Anim. , 11 , 194 – 196 ( 1962. ) ( in Japanese ). [Google Scholar]
  • 14. ) Staats , J.Standardized nomenclature for inbred strains of mice: seventh listing . Cancer Res. , 40 , 2083 – 2128 ( 1980. ). [PubMed] [Google Scholar]
  • 15. ) Moore , R. , Dixon , M. , Smith , R. , Peters , G. and Dickson , C.Complete nucleotide sequence of a milk‐transmitted mouse mammary tumor virus: two frameshift suppression events are required for translation of gag and pol . J. Virol. , 61 , 480 – 490 ( 1987. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 16. ) Eicher , E. M. and Lee , B. K.The NXSM recombinant inbred strains of mice: genetic profile for 58 loci including the Mtv proviral loci . Genetics , 125 , 431 – 446 ( 1990. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 17. ) Lee , B. K. and Eicher , E. M.Segregation patterns of endogenous mouse mammary tumor viruses in five recombinant inbred strain sets . J. Virol. , 64 , 4568 – 4572 ( 1990. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 18. ) Siracusa , L. D. , Jenkins , N. A. and Copeland , N. G.Identification and applications of repetitive probes for gene mapping in the mouse . Genetics , 127 , 169 – 179 ( 1991. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 19. ) Prochazka , M. and Leiter , E. H.Identification of a novel mouse mammary tumor proviral locus (Mtv‐31. on chromosome Y . Mouse Genome , 87 , 111 ( 1990. ). [Google Scholar]
  • 20. ) Tsubura , A. , Ueda , S. , Shikata , N. , Morii , S. and Tanaka , H.Immunohistochemical expression of mammary tumor virus antigens in mammary glands of virgin mice, in relation to Mtv genes . Acta Pathol. Jpn. , 36 , 15 – 22 ( 1986. ). [DOI] [PubMed] [Google Scholar]
  • 21. ) Bentvelzen , P.The biology of the mouse mammary tumor virus . Int. Rev. Exp. Pathol. , 11 , 259 – 297 ( 1972. ). [PubMed] [Google Scholar]
  • 22. ) Teramoto , Y. A. , Dufe , D. and Scholm , J.Multiple antigenic determinants on the major surface glycoprotein of murine mammary tumor viruses . Proc. Natl. Acad. Sci. USA , 74 , 3564 – 3568 ( 1977. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 23. ) Gautsch , J. W. , Lerner , R. , Howard , D. , Teramoto , Y. A. and Schlom , J.Strain‐specific markers for the major structural proteins of highly oncogenic murine mammary tumor viruses by tryptic peptide analyses. J . Virol. , 27 , 688 – 699 ( 1978. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 24. ) Leigler , T. J. and Blair , P. B.Direct detection of exogenous mouse mammary tumor virus sequences in lymphoid cells of BALB/cfC3H female mice . J. Virol , 59 , 159 – 162 ( 1986. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 25. ) Tsubura , A. , Inaba , M. , Imai , S. , Murakami , A. , Oyaizu , N. , Yasumizu , R. , Ohnishi , Y. , Tanaka , H. , Morii , S. and Ikehara , S.Intervention of T‐cells in transportation of mouse mammary tumor virus (milk factor) to mammary gland cells in vivo . Cancer Res. , 48 , 6555 – 6559 ( 1988. ). [PubMed] [Google Scholar]
  • 26. ) Colombo , M. P. , Melvold , R. W. and Wettstein , P. J.Inheritance of mutant histocompatibility gene and a new mammary tumor virus genome in the B6.KH‐84 mouse strain . Immunogenetics , 26 , 99 – 104 ( 1987. ). [DOI] [PubMed] [Google Scholar]
  • 27. ) Cohen , J. C. and Varmus , H. E.Endogenous mammary tumor virus DNA varies among wild mice and segregates during inbreeding . Nature , 278 , 418 – 423 ( 1979. ). [DOI] [PubMed] [Google Scholar]
  • 28. ) Steffen , D. L. , Taylor , B. A. and Weinberg , R. A.Continuing germ line integration of AKV proviruses during the breeding of AKR mice and derivative recombinant inbred strains . J. Virol. , 42 , 165 – 175 ( 1982. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 29. ) Hiai , H. , Buma , Y. O. , Ikeda , H. , Moriwaki , K. and Nishizuka , Y.Epigenetic control of endogenous ecotropic virus expression in SL/Ni mice. J . Natl. Cancer Inst. , 79 , 781 – 787 ( 1987. ). [PubMed] [Google Scholar]
  • 30. ) Gray , D. A. , Lee Chan , E. C. M. , Maclnnes , J. I. and Morris , V. L.Restriction endonuclease map of endogenous mouse mammary tumor virus loci in GR, DBA, and NFS mice , Virology , 148 , 237 – 242 ( 1986. ). [DOI] [PubMed] [Google Scholar]

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