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Japanese Journal of Cancer Research : Gann logoLink to Japanese Journal of Cancer Research : Gann
. 1991 Feb;82(2):206–212. doi: 10.1111/j.1349-7006.1991.tb01830.x

Release of Esterase from Murine Lymphokine‐activated Killer Cells in Antibody‐dependent Cellular Cytotoxic Reaction

Kazunori Kato 1, Toshinori Agatsuma 1, Toshifumi Tanabe 1, Takashi Masuko 1, Yoshiyuki Hashimoto 1,✉
PMCID: PMC5918378  PMID: 1900824

Abstract

Release of granule enzyme(s) (BLT esterase) in the antibody dependent lymphokine‐activated killer (LAK) cell‐mediated cytotoxic reaction (LAK ADCC) was studied using LAK cells induced from murine splenocytes and thymocytes, various human tumor cells and relevant monoclonal antibodies (mAbs) to the tumor cells. BLT esterase was not significantly released from LAK cells in direct LAK cell‐mediated cytotoxic reactions (LAK CMC). However, cultures of LAK cells and IgG‐coated target tumor cells resulted in release of the enzyme concomitantly with target cell lysis, although esterase release proceeded faster than target cell lysis. Anti‐LFA‐1 mAb showed an inhibitory effect on LAK CMC but not on either LAK ADCC or BLT esterase release in the ADCC. These results indicate that exocytosis of granule enzyme from LAK cells is triggered by stimulation of Fc receptor on LAK cells and that LAK CMC and LAK ADCC differ in their lytic mechanism in terms of the release of BLT esterase.

Keywords: Antibody‐dependent cellular cytotoxicity, Lymphokine‐activated killer cell, Esterase, Fc receptor, Lymphocyte function‐associated antigen 1

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