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Japanese Journal of Cancer Research : Gann logoLink to Japanese Journal of Cancer Research : Gann
. 1991 Apr;82(4):371–375. doi: 10.1111/j.1349-7006.1991.tb01857.x

Chromosomal Insertion and Amplification of Human Papillomavirus 16 DNA Sequences in a Cell Line of Argyrophil Small Cell Carcinoma of the Uterine Cervix

Tada‐aki Hori 1, Hiroshi Ichimura 2, Masako Minamihisamatsu 3, Ei‐ichi Takahashi 1, Masatake Yamauchi 1, Yuko Hama 1, Osamu Kurimura 2, Masato Yamasaki 4, Takashi Kurimura 5
PMCID: PMC5918448  PMID: 1646197

Abstract

The chromosomal location of human papillomavirus (HPV) 16 DNA sequences integrated in a cell line derived from argyrophil small cell carcinoma of the uterine cervix was determined by means of fluorescence in situ hybridization (FISH). The HPV 16 DNA sequences were integrated near a fragile site and the location of the c‐myc oncogene at 8q24.1. Amplification of the integrated viral sequences resulted in an abnormally banded region. The amplified HPV 16 DNA sequences were also detected in every interphase nucleus by FISH.

Keywords: Cervial carcinoma, Human papillomavirus (HPV 16), Fluorescence in situ hybridization

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