Abstract
In order to study the role of CD34+ cells in hematological recovery following bone marrow transplantation (BMT), bone marrow cells stained with HPCA‐1 (CD34) and MY‐9 (CD33) monoclonal antibodies were analyzed by using a fluorescence‐activated cell sorter on or about days 14 and 28, as well as at later times, following BMT in 6 recipients. Single cell cultures of CD34+ cells were also performed to evaluate their in vitro hematopoietic function. CD34+ cells were detectable in bone marrow cells on day 14. More than 80% of CD34+ cells co‐expressed the CD33 antigen, and macrophage (Mac) colony‐forming cells predominated among total colony‐forming cells of CD34+ cells. In normal bone marrow cells, CD34+, CD33+ cells amounted to about 40% of CD34+ cells, and the incidences of erythroid bursts, granulocyte/macrophage (GM) colonies, and Mac colonies were similar to each other. After more than 10 weeks, CD34+, CD33− cells gradually recovered, as erythroid burst colony‐forming cells increased following GM colony‐forming cells. This phenomenon was well‐correlated with the time course of peripheral blood cell recovery. CD34+, CD33+ cells as committed progenitors and CD34+, CD33− cells as multipotent stem cells have distinctive biological behaviors in BMT.
Keywords: Bone marrow transplantation, CD34+ cells, Hematopoietic stem cells
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REFERENCES
- 1. ) Civin , C. I. , Strauss , L. C , Brovall , C. , Fackler , M. J. , Schwartz , J. F. and Shaper , J. H.Antigenic analysis of hematopoiesis. III. A hematopoietic progenitor cells surface antigen defined by a monoclonal antibody raised against KG‐1a cells . J Immunol. , 133 , 157 – 165 ( 1984. ). [PubMed] [Google Scholar]
- 2. ) Civin , C. I. , Banquerigo , M. L. , Strauss , L. C. and Loken , M. R.Antigenic analysis of hematopoiesis. VI. Flow cytometric characterization of My‐10‐positive progenitor cells in normal human bone marrow , Exp. Hematol. , 15 , 10 – 17 ( 1987. ). [PubMed] [Google Scholar]
- 3. ) Berenson , R. J. , Andrews , R. G. , Bensinger , W. L , Kalamasz , D. , Knitter , G. , Buckner , C. D. and Bernstein , I. D.Antigen CD34+ marrow cells engraft lethally irradiated baboons . J. Clin. Invest. , 81 , 951 – 955 ( 1988. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
- 4. ) Siena , S. , Bregni , M. , Brando , B. , Ravagnani , F. , Bonadonna , G. and Gianni , A. M.Circulation of CD34+ hematopoietic stem cells in the peripheral blood of high‐dose cyclophosphamide‐treated patients: enhancement by intravenous recombinant human granulocyte‐macrophage colony‐stimulating factor . Blood , 74 , 1905 – 1914 ( 1989. ). [PubMed] [Google Scholar]
- 5. ) Andrews , R. G. , Singer , J. W. and Bernstein , I. D.Precursors of colony‐forming cells in humans can be distinguished from colony‐forming cells by expression of the CD33 and CD34 antigens and light scatter properties . J. Exp. Med. , 169 , 1721 – 1731 ( 1989. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
- 6. ) Ema , H. , Suda , T. , Miura , Y. and Nakauchi , H.Colony formation of clone‐sorted human hematopoietic progenitors . Blood , 75 , 1941 – 1946 ( 1990. ). [PubMed] [Google Scholar]
- 7. ) Moore , W. and Kantz , R.Data analysis in flow cytometry . In “ The Handbook of Experimental Immunology ”, 4th Ed. , ed. Weir D. M. , Herzenberg L. A. , Blackwell C. and Herzenberg L. A. , pp. 30.1 ( 1986. ). Blackwell; , Edinburgh , Scotland . [Google Scholar]
- 8. ) Messner , H. A. , Jamal , N. and Izaguirre , C.The growth of large megakaryocyte colonies from human bone marrow . J. Cell. Physiol. 1 ( suppl. ), 45 – 51 ( 1982. ). [DOI] [PubMed] [Google Scholar]
- 9. ) Kimura , H. , Burstein , A. S. , Thorning , D. , Powell , J. S. , Harker , L. A. , Fialkow , P. J. and Adamson , J. W.Human megakaryocytic progenitors (CFU‐M) assayed in methylcellulose: physical characteristics and requirements for growth . J Cell Physiol. , 118 , 87 – 96 ( 1984. ). [DOI] [PubMed] [Google Scholar]
- 10. ) Rambaldi , A. , Young , D. C. and Griffin , J. D.Expression of the M‐CSF (CSF‐1) gene by human monocytes . Blood , 69 , 1409 – 1413 ( 1987. ). [PubMed] [Google Scholar]
- 11. ) Lee , S. H. , Crocker , P. R. , Westaby , S. , Key , N. , Mason , D. Y. and Gordon , S.Isolation and immunocytochemical characterization of human bone marrow stromal macro‐phages in hemopoietic cultures . J. Exp. Med. , 168 , 1193 – 1198 ( 1988. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
- 12. ) Jones , R. J. , Celano , P. , Sharkis , S. J. and Sensenbrenner , L. L.Two phases of engraftment established by serial bone marrow transplantation in mice . Blood , 73 , 397 – 401 ( 1989. ). [PubMed] [Google Scholar]
- 13. ) Metcalf , D.Hemopoietic growth factors 1 . Lancet , i , 825 – 827 ( 1989. ). [DOI] [PubMed] [Google Scholar]