Abstract
Availability of accurate prognostic factors is vital in making decisions on cancer therapy. We have measured the cytosolic contents of phosphoethanolamine and ethanolamine in tumor tissues of 53 breast cancer patients in an attempt to explore the possibility that these amines could be used as prognostic indicators. The levels of phosphoethanolamine and ethanolamine were determined by high‐performance liquid chromatography. The ratios of the molar quantity of these amines or amino acids to that of alanine plus tyrosine, which eluted as a single peak, were used to analyze and compare the results among different tumor samples. The results indicated that the values for phosphoethanolamine or ethanolamine varied significantly more than the values for amino acids, such as glycine plus threonine or glutamine plus serine (these amino acids were eluted as single peaks, respectively). The values for phosphoethanolamine, ethanolamine, and phosphoethanolamine plus ethanolamine were analyzed in relation to several commonly used prognostic factors of breast disease. The results indicated that groups having higher mitotic indices had significantly higher values for phosphoethanolamine or phosphoethanolamine plus ethanolamine than the group having lower mitotic indices. As the stage of the disease increased, the values for phosphoethanolamine plus ethanolamine also seemed to become higher. No correlation, however, was observed between steroid hormone receptor positive and negative groups or between positive and negative groups with regard to involved axillary lymph nodes. The content of phosphoethanolamine or phosphoethanolamine plus ethanolamine in cytosol therefore seems to be correlated with some prognostic indicators.
Keywords: Phosphoethanolamine, Ethanolamine, Breast cancer
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REFERENCES
- 1. ) Stoll , B. A.Prognostic indices in breast cancer . In “ Pointers to Cancer Prognosis,’ ed. Stoll B. A. , pp. 195 – 229 ( 1987. ). Martinus Nijhoff Publishers; , Dordrecht , Netherlands . [Google Scholar]
- 2. ) Page , D. L. and Anderson , T. J. “ Diagnostic Histopathology of the Breast ” ( 1987. ). Churchill Livingstone; , Edinburgh, London, Melbourne and New York . [Google Scholar]
- 3. ) Clark , G. M. and McGuire , W. L.Steroid receptors and other prognostic factors in primary breast cancer . Semin. Oncol , 15 ( Suppl. 1 ), 20 – 25 ( 1988. ). [PubMed] [Google Scholar]
- 4. ) Contesso , G. , Saccanijotti , G. and Bonadonna , G.Tumor grade as a prognostic factor in primary breast cancer . Eur. J. Cancer Clin. Oncol. , 25 , 403 – 409 ( 1989. ). [DOI] [PubMed] [Google Scholar]
- 5. ) Rochefort , H.Cathepsin D in breast cancer . Breast Cancer Res. Treat. , 16 , 3 – 13 ( 1990. ). [DOI] [PubMed] [Google Scholar]
- 6. ) Kano‐Sueoka , T. and Errick , J. E.Effects of phosphoethanolamine and ethanolamine on growth of mammary carcinoma cells in culture . Exp. Cell Res. , 136 , 137 – 145 ( 1981. ). [DOI] [PubMed] [Google Scholar]
- 7. ) Kano‐Sueoka , T. and King , D.Role of ethanolamine and phosphatidylethanolamine in proliferation of mammary epithelial cells . In “ Growth and Differentiation of Mammary Epithelial Cells in Culture ,” ed. Enami J. and Ham R. G. , pp. 39 – 58 ( 1987. ). Japan Scientific Societies Press; , Tokyo . [Google Scholar]
- 8. ) Kano‐Sueoka , T. , Errick , J. E. , King , D. and Walsh , L. A.Phosphatidylethanolamine synthesis in ethanolamine‐re‐sponsive and ‐nonresponsive cells in culture . J. Cell. Physiol. , 117 , 109 – 115 ( 1983. ). [DOI] [PubMed] [Google Scholar]
- 9. ) Kano‐Sueoka , T. and King , D.Phosphatidylethanolamine biosynthesis in rat mammary carcinoma cells that require and do not require ethanolamine for proliferation . J. Biol. Chem. , 262 , 6074 – 6081 ( 1987. ). [PubMed] [Google Scholar]
- 10. ) Ellison , D. W. , Beal , M. F. and Martin , J. B.Amino acid neurotransmitters in postmortem human brain analyzed by high performance liquid chromatography with electrochemical detection . J, Neurosci. Methods , 19 , 305 – 315 ( 1987. ). [DOI] [PubMed] [Google Scholar]
- 11. ) Ellison , D. W. , Beal , M. F. and Martin , J. B.Phosphoethanolamine and ethanolamine are decreased in Alzheimer's disease and Huntington's disease . Brain Res. , 417 , 389 – 392 ( 1987. ). [DOI] [PubMed] [Google Scholar]