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Japanese Journal of Cancer Research : Gann logoLink to Japanese Journal of Cancer Research : Gann
. 1992 Feb;83(2):153–158. doi: 10.1111/j.1349-7006.1992.tb00080.x

Production of Multiple Growth Factors by a Newly Established Human Thyroid Carcinoma Cell Line

Kaoru Tohyama 1,✉, Yataro Yoshida 2, Kensaku Ohashi 4, Emiko Sano 4, Hisataka Kobayashi 3, Keigo Endo 3, Masanobu Naruto 4, Toru Nakamura 1
PMCID: PMC5918779  PMID: 1372885

Abstract

A multiple growth factor‐producing tumor cell line (NIM‐1) was newly established from a patient with thyroid cancer and remarkable neutrophilia. NIM‐1 cells also caused severe neutrophilia in nude mice bearing tumors. NIM‐1‐conditioned medium (NIM‐1CM) contained activities that supported not only granulocyte, macrophage and eosinophil colony formation of human bone marrow cells but also the growth of colony‐stimulating factor (CSF)‐dependent cell lines, NFS60‐KX and TF‐1. Northern blot hybridization analysis revealed the constitutive expression of granulocyte‐CSF (G‐CSF), granulocyte/macrophage‐CSF (GM‐CSF) and interleukin(IL)‐6 mRNAs in NIM‐1 cells. Enzyme‐linked immunosorbent assays (ELISA) using NIM‐1CM also confirmed the production of IL‐la and a small amount of IL‐1β besides G‐CSF, GM‐CSF and IL‐6 in NIM‐1 cells. In addition, unexpected production of IL‐11 in NIM‐1 cells was detected by northern blot hybridization analysis and by bioassay using an IL‐11‐dependent cell line. Therefore, NIM‐1 cell line is shown to produce multiple cytokines including potentially megakaryopoietic growth factors such as GM‐CSF, IL‐6 and IL‐11.

Keywords: Key words, Thyroid carcinoma cell line, Hemopoietic growth factor, Leukocytosis

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