Abstract
Monoclonal antibody (mAb) NU7‐99 reacted with only B6RV2 cells, not with 28 other leukemia cell lines, fibroblasts or normal tissues. Biochemical analyses of the unique antigen on B6RV2 cells that reacted with NU7–99 mAb indicated its relationship to xenotropic murine leukemia virus gp70. The antigen that reacted with NU7‐99 mAb was extracted from the surface of B6RV2 cells with n‐butanol and purified by ion‐exchange chromatography and affinity chromatography. Growth of B6RV2 tumors in semi‐syngeneic mice was inhibited by immunization of the mice with a purified preparation of this unique antigen.
Keywords: Key words, Murine leukemia, Unique antigen, Murine leukemia virus, Tumor rejection antigen
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REFERENCES
- 1. ) Morishita , H. , Shiku , H. , Horibe , K. , Obata , Y. , Stockert , E. , Oettgen , H. F. , Old , L. J. and Yamada , K.Cell surface antigens of murine leukemias induced by radiation leukemia virus. Recognition of individually distinct cell surface antigens by cytotoxic T cells on leukemias expressing cross reactive transplantation antigens . J, Exp, Med. , 163 , 452 – 457 ( 1986. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
- 2. ) Gross , L.Intradermal immunization of C3H mice against a sarcoma that originated in an animal of the same line . Cancer Res. , 3 , 326 – 333 ( 1943. ). [Google Scholar]
- 3. ) Foley , E. J.Antigenic properties of methylcholanthrene‐induced tumors in mice of the strain of origin . Cancer Res. , 13 , 835 – 837 ( 1953. ). [PubMed] [Google Scholar]
- 4. ) Prehn , R. T. and Main , J. M.Immunity to methyl‐cholanthrene‐induced sarcomas . Natl. Cancer Inst. , 18 , 769 – 778 ( 1957. ). [PubMed] [Google Scholar]
- 5. ) Klein , G. , Sjogren , H. O. , Klein , E. and Hellstrom , K. E.Demonstration of resistance against methylcholanthrene‐induced sarcomas in the primary autochthonous host . Cancer Res. , 20 , 1561 – 1572 ( 1960. ). [PubMed] [Google Scholar]
- 6. ) Nakayama , E. , Uenaka , A. , Stockert , E. and Obata , Y.Detection of a unique antigen on radiation leukemia virus‐induced leukemia B6RV2 . Cancer Res. , 44 , 5138 – 5144 ( 1984. ). [PubMed] [Google Scholar]
- 7. ) Nakayama , E. , Uenaka , A. , DeLeo , A. B. , Stockert , E. , Obata , Y. , Ueda , R. and Inui , Y.Molecular basis of a unique tumor antigen of radiation leukemia virus‐induced leukemia B6RV2: its relation to MuLV gp70 of xenotropic class . J. Immunol. , 136 , 3502 – 3507 ( 1986. ). [PubMed] [Google Scholar]
- 8. ) Stockert , E. , DeLeo , A. B. , O'Donnell , P. V. , Obata , Y. and Old , L. J.G(AKSL2): a new cell surface antigen of the mouse related to the dualtropic mink cell focus‐inducing class of murine leukemia virus detected by naturally occurring antibody . J. Exp. Med. , 149 , 200 – 215 ( 1979. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
- 9. ) Laemmli , U. K.Cleavage of structural proteins during the assembly of the head of bacteriophage T4 . Nature , 227 , 680 – 685 ( 1970. ). [DOI] [PubMed] [Google Scholar]
- 10. ) DeLeo , A. B. , Shiku , H. , Takahashi , T. , John , M. and Old , L. J.Cell surface antigens of chemically induced sarcomas of the mouse. 1. Murine leukemia virus‐related antigens and alloantigens on cultured fibroblasts and sarcoma cells: description of a unique antigen on BALB/c Meth A sarcoma . J. Exp. Med. , 146 , 720 – 734 ( 1977. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
- 11. ) Srivastava , P. K. , DeLeo , A. B. and Old , L. J.Tumor rejection antigens of chemically induced sarcomas of inbred mice. Proc . Natl. Acad. Sci. USA , 83 , 3407 – 3411 ( 1986. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
- 12. ) Srivastava , P. K. , Chen , Y. T. and Old , L. J.5′‐Structural analysis of genes encoding polymorphic antigens of chemically induced tumors . Proc. Natl. Acad. Sci. USA , 84 , 3807 – 3811 ( 1987. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
- 13. ) DuBois , G. C. , Law , L. W. and Appella , E.Purification and biochemical properties of tumor‐associated transplantation antigens from methylcholanthrene‐induced murine sarcomas . Proc. Natl. Acad. Sci. USA , 79 , 7669 – 7673 ( 1982. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
- 14. ) DuBois , G. C. and Law , L. W.Biochemical characterization and biologic activities of 82‐ and 86‐kDa tumor antigens isolated from a methylcholanthrene‐induced sarcoma, CII‐7 . Int. J. Cancer , 37 , 925 – 931 ( 1986. ). [DOI] [PubMed] [Google Scholar]
- 15. ) LeGrue , S. J. , Kahan , B. D. and Pellis , N. R.Extraction of a murine tumor‐specific transplantation antigen with 1‐butanol. 1, Partial purification by isoelectric focusing . J. Natl. Cancer Inst. , 65 , 191 – 196 ( 1980. ). [PubMed] [Google Scholar]
- 16. ) LeGrue , S. J. , Pellis , N. R. , Riley , L. B. and Kahan , B. D.Biochemical characterization of 1‐butanol‐extracted murine tumor‐specific transplantation antigens . Cancer Res. , 45 , 3164 – 3172 ( 1985. ). [PubMed] [Google Scholar]
- 17. ) Plata , F. , Langlade‐Demoyen , P. , Abastado , J. P. , Berbar , T. and Kourilsky , P.Retrovirus antigens recognized by cytolytic T lymphocytes activate tumor rejection in vivo . Cell , 48 , 231 – 240 ( 1987. ). [DOI] [PubMed] [Google Scholar]
- 18. ) Nakayama , E. , Shiku , H. , Takahashi , T. , Oettgen , H. F. and Old , L. J.Definition of a unique cell surface antigen of mouse leukemia RL1 by cell‐mediated cytotoxicity . Proc. Natl. Acad. Sci. USA , 76 , 3486 – 3490 ( 1979. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
- 19. ) Duprez , V. , Gomard , E. and Levy , J. P.Cell‐mediated antitumor response in the RLl system. 1. T nature, H‐2Ddinvolvement and antigenic specificity of the effector cells . Eur. J. Immunol , 8 , 650 – 655 ( 1978. ). [DOI] [PubMed] [Google Scholar]