Skip to main content
NCBI home page
Japanese Journal of Cancer Research : Gann logoLink to Japanese Journal of Cancer Research : Gann
. 1992 Jul;83(7):671–675. doi: 10.1111/j.1349-7006.1992.tb01963.x

Low Frequency of Rearrangements of the ret and trk Proto‐oncogenes in Japanese Thyroid Papillary Carcinomas

Worawidh Wajjwalku 1, Shigeo Nakamura 3, Yasuhisa Hasegawa 4, Koichi Miyazaki 2, Yasuyuki Satoh 2, Hiroomi Funahashi 2, Mutsushi Matsuyama 1, Masahide Takahashi 1,✉
PMCID: PMC5918939  PMID: 1381340

Abstract

We investigated the frequency of rearrangements of the ret and trk proto‐oncogenes in Japanese thyroid tumors. DNAs from 38 thyroid papillary carcinomas and 14 follicular adenomas were analyzed by Southern blotting. Rearrangements of the ret and trk proto‐oncogenes were detected in one and two papillary carcinomas, respectively, but not in follicular adenomas. Analysis by a reverse transcriptase‐polymerase chain reaction method showed that the ret rearrangement‐positive tumor contained the PTC/refTPCchimeric transcript, which was reported to be found specifically in thyroid tumors and adenomatous goiter. We also found that rearranged mRNA of the trk proto‐oncogene was expressed at high levels in one of two trk rearrangement‐positive tumors. Our results indicated that the frequency of rearrangements of these proto‐oncogenes in Japanese papillary carcinomas was much lower than that in Italian patients.

Keywords: Key words, ret and trk proto‐oncogenes, Rearrangement, Thyroid papillary carcinoma

Full Text

The Full Text of this article is available as a PDF (271.9 KB).

REFERENCES

  • 1. ) Leder , P. , Batty , J. , Lenoir , G. , Moulding , C. , Murphy , W. , Potter , H. , Stewart , T. and Taub , R.Translocations among antibody genes in human cancer . Science , 222 , 765 – 771 ( 1983. ). [DOI] [PubMed] [Google Scholar]
  • 2. ) Tsujimoto , Y. , Cossman , J. , Jaffee , E. and Croce , C. M.Involvement of the bcl‐2 gene in human follicular lymphoma . Science , 228 , 1440 – 1443 ( 1985. ). [DOI] [PubMed] [Google Scholar]
  • 3. ) Konopka , J. B. , Watanabe , S. M. and Witte , O. N.An alteration of the human c‐abl protein in K562 leukemia cells unmasks associated tyrosine kinase activity . Cell , 37 , 1035 – 1042 ( 1984. ). [DOI] [PubMed] [Google Scholar]
  • 4. ) Takahashi , M. , Ritz , J. and Cooper , G. M.Activation of a novel human transforming gene, ret, by DNA rearrangement . Cell , 42 , 581 – 588 ( 1985. ). [DOI] [PubMed] [Google Scholar]
  • 5. ) Takahashi , M. and Cooper , G. M.ret transforming gene encodes a fusion protein homologous to tyrosine kinases . Mol. Cell. Biol , 7 , 1378 – 1385 ( 1987. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 6. ) Martin‐Zanca , D. , Hughes , S. H. and Barbacid , M.A human oncogene formed by the fusion of truncated tropomyosin and protein tyrosine kinase sequences . Nature , 319 , 743 – 748 ( 1986. ). [DOI] [PubMed] [Google Scholar]
  • 7. ) Grieco , M. , Santoro , M. , Berlingieri , M. T. , Melillo , R. M. , Donghi , R. , Bongarzone , I. , Pierotti , M. A. , Delia Porta , G. , Fusco , A. and Vecchio , G.PTC is a novel rearranged form of the ret proto‐oncogene and is frequently detected in vivo in human thyroid papillary carcinomas . Cell , 60 , 557 – 563 ( 1990. ). [DOI] [PubMed] [Google Scholar]
  • 8. ) Bongarzone , I.Pierotti , M. A. , Monzini , N. , Mondellini , P. , Manenti , G. , Donghi , R. , Pilotti , S. , Grieco , M. , Santoro , M. , Fusco , A. , Vecchio , G. and Delia Porta , G.High frequency of activation of tyrosine kinase oncogenes in human papillary thyroid carcinoma . Oncogene , 4 , 1457 – 1462 ( 1989. ). [PubMed] [Google Scholar]
  • 9. ) Ishizaka , Y. , Ushijima , T. , Sugimura , T. and Nagao , M.cDNA cloning and characterization of ret activated in a human papillary thyroid carcinoma cell line . Biochem. Biophys. Res. Commun. , 168 , 402 – 408 ( 1990. ). [DOI] [PubMed] [Google Scholar]
  • 10. ) Radice , P. , Sozzi , G. , Miozzo , M. , De Benedetti , V. , Cariani , T. , Bongarzone , I. , Spurr , N. K. , Pierotti , M. A. and Delia Porta , G.The human tropomyosin gene involved in the generation of the TRK oncogene maps to chromosome Iq31 . Oncogene , 6 , 2145 – 2148 ( 1991. ). [PubMed] [Google Scholar]
  • 11. ) Ishizaka , Y. , Itoh , F. , Tahira , T. , Ikeda , I. , Sugimura , T. , Tucker , J. , Fertitta , A. , Carrano , A. V. and Nagao , M.Human ret proto‐oncogene mapped to chromosome 10qll.2 . Oncogene , 4 , 1519 – 1521 ( 1989. ). [PubMed] [Google Scholar]
  • 12. ) Sozzi , G. , Pierotti , M. A. , Miozzo , M. , Donghi , R. , Radice , P. , De Benedetti , V. , Grieco , M. , Santoro , M. , Fusco , A. , Vecchio , G. , Mathew , C. G. P. , Ponder , B. A. J. , Spurr , N. K. , and Delia Porta , G.Refined localization to contiguous regions on chromosome 10q of the two gene (H4 and RET) that form the oncogenic sequence PTC . Oncogene , 6 , 339 – 342 ( 1991. ). [PubMed] [Google Scholar]
  • 13. ) Miozzo , M. , Pierotti , M. A. , Sozzi , G. , Radice , P. , Bongarzone , I. , Spurr , N. K. and Delia Porta , G.Human TRK proto‐oncogene maps to chromosome Iq32‐q41 . Oncogene , 5 , 1411 – 1414 ( 1990. ). [PubMed] [Google Scholar]
  • 14. ) Ishizaka , Y. , Kobayashi , S. , Ushijima , T. , Hirohashi , S. , Sugimura , T. and Nagao , M.Detection of refTPC/PTC transcripts in thyroid adenomas and adenomatous goiters by an RT‐PCR method . Oncogene , 6 , 1667 – 1672 ( 1991. ). [PubMed] [Google Scholar]
  • 15. ) Takahashi , M. , Inaguma , Y. , Hiai , H. and Hirose , F.Developmentally regulated expression of a human “finger”‐containing gene encoded by the 5′ half of the ret transforming gene . Mol. Cell. Biol , 8 , 1853 – 1856 ( 1988. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 16. ) Martin‐Zanca , D. , Oskam , R. , Mitra , G.Copeland , T. and Barbacid , M.Molecular and biochemical characterization of the human trk proto‐oncogene . Mol Cell. Biol , 9 , 24 – 33 ( 1989. ). [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Japanese Journal of Cancer Research : Gann are provided here courtesy of Wiley

RESOURCES