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Japanese Journal of Cancer Research : Gann logoLink to Japanese Journal of Cancer Research : Gann
. 1992 Sep;83(9):933–936. doi: 10.1111/j.1349-7006.1992.tb02003.x

Absence of ras Family Point Mutations at Codons 12, 13 and 61 in N‐Ethyl‐N‐hydroxyethylnitrosamine‐ or N‐Nitrosomorpholine‐induced Renal Cell Tumors in Rats

Kazuyuki Matsumoto 1, Hiroyuki Tsuda 1, Teruhiko Iwase 1, Mitsuya Ito 1, Yoshihisa Nishida 2, Fumitaka Oyama 3, Koiti Titani 3, Toshikazu Ushijima 4, Minako Nagao 4, Iwao Hirono 1
PMCID: PMC5918969  PMID: 1429202

Abstract

The prevalence of Ki‐ras, Ha‐ras and N‐ras point mutation within exons 1 and 2 was studied in 17 cases of renal cell tumors (8 carcinomas and 9 adenomas) induced by N‐ethyl‐N‐hydroxyethylnitrosamine or N‐nitrosomorpholine. DNA samples prepared from acetone‐fixed, paraffin‐embedded tissues were amplified by means of the polymerase chain reaction, and point mutations at codons 12, 13 and 61 were analyzed by direct sequence methods with oligonucleotide primers. No mutations were detected in any of the renal tumors. The results thus indicated that ras family point mutation is not necessary for kidney tumor development in rats, supporting the view that ras mutations may not be generally relevant to neoplastic development in various organs in different species.

Keywords: Key words, ras Point mutation, Rat renal cell tumor, Nitrosamine‐induced tumor, Polymerase chain reaction

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