Skip to main content
PMC home page
Japanese Journal of Cancer Research : Gann logoLink to Japanese Journal of Cancer Research : Gann
. 1993 Oct;84(10):1010–1014. doi: 10.1111/j.1349-7006.1993.tb02793.x

Influence of Esculetin on Incidence, Proliferation, and Cell Kinetics of Mammary Carcinomas Induced by 7,12‐Dimethylbenz[α]anthracene in Rats on High‐ and Low‐fat Diets

Masakuni Noguchi 1,2,✉, Hirohisa Kitagawa 1, Itsuo Miyazaki 1, Yuji Mizukami 3
PMCID: PMC5919062  PMID: 8226274

Abstract

The effects of a high‐fat diet and esculetin were investigated on 7,12‐dimethylbenz[α]anthracene (DMBA)‐induced mammary carcinogenesis in female Sprague‐Dawley rats. Rats were given a 5‐mg dose of DMBA. Seven days later, they were fed either a high‐fat (20% soybean oil) or low‐fat (0.5% soybean oil) diet. A half of the rats received diets containing 0.03% esculetin. Esculetin significantly inhibited tumor incidence, growth and cell kinetics of the tumor in the rats fed the high‐fat and the low‐fat diets. Our findings indicate that DMBA‐induced mammary tumorigenesis is affected by lipoxygenase products.

Keywords: Esculetin; High dietary fat; Mammary carcinoma; 7,12‐Dimethylbenz[α]‐anthracene

Full Text

The Full Text of this article is available as a PDF (293.9 KB).

REFERENCES

  • 1).Hirayama , T.Epidemiology of breast cancer with special reference to the role of diet . Prev. Med. , 7 , 173 – 195 ( 1978. ). [DOI] [PubMed] [Google Scholar]
  • 2).Wynder , E. L. , Rose , D. P. and Cohen , L. A.Diet and breast cancer in causation and therapy . Cancer , 58 , 1804 – 1813 ( 1986. ). [DOI] [PubMed] [Google Scholar]
  • 3).Hill , P. and Wynder , E. L.Comparison of mammary adipose fatty acid composition in Japanese and American breast cancer patients . Eur. J. Cancer Clin. Oncol. , 23 , 407 – 410 ( 1987. ). [DOI] [PubMed] [Google Scholar]
  • 4).Kritchesky , D.Nutrition and breast cancer . Cancer , 66 , 1321 – 1325 ( 1990. ). [DOI] [PubMed] [Google Scholar]
  • 5).Welsch , C. W. , House , J. L. , Herr , B. L. , Eliasberg , S. J. and Welsch , M. A.Enhancement of mammary carcinogenesis of high levels of dietary fat: a phenomenon dependent on ad libitum feeding . J. Natl. Cancer Inst. , 82 , 1615 – 1620 ( 1990. ). [DOI] [PubMed] [Google Scholar]
  • 6).Welsch . C. W. and Aylsworth , C. F.Enhancement of murine mammary tumorigenesis by feeding high levels of dietary fat: a hormonal mechanism ? J. Natl. Cancer Inst. , 70 , 215 – 221 ( 1983. ). [PubMed] [Google Scholar]
  • 7).Carter , C. A. , Milholland , R. J. , Shea , W. and Ip , M. M.Effect of the prostaglandin synthetase inhibitor indomethacin on 7,12‐dimethylbenz(α)anthracene‐induced mammary tumorigenesis in rats fed different levels of fat . Cancer Res. , 38 , 3559 – 3562 ( 1983. ). [PubMed] [Google Scholar]
  • 8).McCormick , D. L. , Madigan , M. J. and Moon , R. C.Modulation of rat mammary carcinogenesis by indomethacin . Cancer Res. , 45 , 1803 – 1808 ( 1985. ). [PubMed] [Google Scholar]
  • 9).McCormick , D. L. and Moon , R. C.Inhibition of mammary carcinogenesis by flurbiprofen, a non‐steroidal anti‐inflammatory agent . Br. J. Cancer , 48 , 859 – 861 ( 1983. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 10).Hillyard , L. A. and Abraham , S.Effect of dietary polyunsaturated fatty acids on growth of mammary adenocarcinomas in mice and rats . Cancer Res. , 39 , 4430 – 4437 ( 1979. ). [PubMed] [Google Scholar]
  • 11).Fulton , A. M.In vivo effects of indomethacin on the growth of murine mammary tumors . Cancer Res. , 44 , 2416 – 2420 ( 1984. ). [PubMed] [Google Scholar]
  • 12).Fulton , A. M. and Heppner , G. H.Relationships of prostaglandin E and natural killer sensitivity to metastatic potential in murine mammary adenocarcinomas . Cancer Res. , 45 , 4779 – 4784 ( 1985. ). [PubMed] [Google Scholar]
  • 13).Kollmorgen , G. M. , King , M. M. , Kosanke , S. D. and Do , C.Influence of dietary fat and indomethacin on the growth of transplantable mammary tumors in rats . Cancer Res. , 43 , 4714 – 4719 ( 1983. ). [PubMed] [Google Scholar]
  • 14).Nagasawa , H. and Naito , T.Inhibition by indomethacin of spontaneous mammary tumorigenesis in SHN mice . Breast Cancer Res. Treat. , 8 , 249 – 255 ( 1986. ). [DOI] [PubMed] [Google Scholar]
  • 15).Hubbard , N. E. , Chapkin , R. S. and Erickson , K. L.Inhibition of growth and linoleate‐enhanced metastasis of a transplantable mouse mammary tumor by indomethacin . Cancer Lett. , 43 , 111 – 120 ( 1988. ). [DOI] [PubMed] [Google Scholar]
  • 16).Carter , C. A. , Ip , M. M. and Ip , C.A comparison of the effects of the prostaglandin synthesis inhibitors indomethacin and carprofen on 7,12‐dimethylbenz(α)anthracene‐induced mammary tumorigenesis in rats fed different amounts of essential fatty acid . Carcinogenesis , 10 , 1369 – 1374 ( 1989. ). [DOI] [PubMed] [Google Scholar]
  • 17).Noguchi , M. , Taniya , T. , Koyasaki , N. , Kumaki , T. , Miyazaki , I. and Mizukami , Y.Effects of the prostaglandin synthetase inhibitor indomethacin on tumorigenesis, tumor proliferation, cell kinetics, and receptor contents of 7,12‐dimethylbenz(α)anthracene‐induced mammary carcinoma in Sprague‐Dawley rats fed a high‐or low‐fat diet . Cancer Res. , 51 , 2683 – 2689 ( 1991. ). [PubMed] [Google Scholar]
  • 18).Noguchi , M. and Ohta , N.Influences of high dietary fat and/or indomethacin on tumor proliferation and cell kinetics of 7,12‐dimethyl‐benz(a)anthracence‐induced mammary carcinomas in rats . In vitro , 7 , 53 – 58 ( 1993. ). [PubMed] [Google Scholar]
  • 19).Neichi , T. , Koshihara , Y. and Murota , S.Inhibitory effect of esculetin on 5‐lipoxygenase and leukotriene biosynthesis . Biochim. Biophys. Acta , 753 , 130 – 132 ( 1983. ). [PubMed] [Google Scholar]
  • 20).Gren , R. I. , Greenberg , N. H. and MacDonald , M. M.Protocol for screening chemical agents and natural products against animal tumors and other biological systems . Cancer Chemother. Rep. , 3 , 51 – 61 ( 1972. ). [Google Scholar]
  • 21).Collins , V. P. , Loeffler , R. K. and Tivey , H.Observations on growth rates of human tumors . Am. J. Roentgenol. , 76 , 988 – 1000 ( 1956. ). [PubMed] [Google Scholar]
  • 22).Kuehl , F. A. , Dougherty , H. W. and Ham , E. A.Interactions between prostaglandins and leukotrienes . Biochem. Pharmacol. , 33 , 1 – 5 ( 1984. ). [DOI] [PubMed] [Google Scholar]
  • 23).Kaplan , L. , Weiss , J. and Elsbach , P.Low concentrations of indomethacin inhibit phospholipase A2 of rabbit polymorphonuclear leukocytes . Proc. Natl. Acad. Sci. USA , 75 , 2955 – 2958 ( 1978. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 24).Vanderhoek , J. Y. , Ekborg , S. L. and Bailey , J. M.Effects of cell growth, differentiation and transformation: clinical involvement of leukotrienes . J. Allergy Clin. Immunol. , 74 , 412 – 417 ( 1984. ). [DOI] [PubMed] [Google Scholar]
  • 25).Blomgren , H. and Andersson , G. K.Growth inhibition of human malignant glioma cells in vitro by agents which interfere with biosynthesis of eicosanoids . Anticancer Res. , 12 , 981 – 986 ( 1992. ). [PubMed] [Google Scholar]
  • 26).Lee , P. P. H. and Ip , M. M.Regulation of proliferation of rat mammary tumor cells by inhibitors of cyclooxygenase and lipoxygenase . Prostaglandins Leukotrienes Essent. Fatty Acids , 45 , 21 – 31 ( 1992. ). [DOI] [PubMed] [Google Scholar]
  • 27).Rose , D. P. and Connoly , J. M.Effects of fatty acids and inhibitors of eicosanoid synthesis on the growth of a human breast cancer cell line in culture . Cancer Res. , 50 , 7139 – 7144 ( 1990. ). [PubMed] [Google Scholar]
  • 28).Kragballe , K. , Desjarlais , L. and Voorhees , J. J.Leukotrienes B4, C4 and D4 stimulate DNA synthesis in cultured human epidermal keratinocytes . Br. J. Dermatol. , 113 , 43 – 53 ( 1985. ). [DOI] [PubMed] [Google Scholar]
  • 29).Atluru , D. and Goodwin , J. S.Leukotriene B4 causes proliferation of interleukin 2‐dependent T‐cells in the presence of suboptimal levels of interleukin 2 . Cell Immunol. , 99 , 444 – 452 ( 1986. ). [DOI] [PubMed] [Google Scholar]
  • 30).Snyder , D. S. , Castro , R. and Desforges , J. F.Anti‐proliferative effects of lipoxygenase inhibitors on malignant human hematopoietic cell lines . Exp. Hematol. , 17 , 6 – 9 ( 1989. ). [PubMed] [Google Scholar]
  • 31).Vanderhoek , J. Y. and Bailey , J. M.Activation of a 15‐lipoxygenase/leukotriene pathway in human polymorphonuclear leukocytes by the anti‐inflammatory agent ibuprofen . J. Biol. Chem. , 259 , 6752 – 6756 ( 1984. ). [PubMed] [Google Scholar]
  • 32).Myers , R. F. and Siegal , M. I.Differential effects of anti‐inflammatory drugs on lipoxygenase and cyclooxygenase activities of neutrophils from a reverse passive Arthus reaction . Biochem. Biophys. Res. Commun. , 112 , 586 – 594 ( 1983. ). [DOI] [PubMed] [Google Scholar]
  • 33).Abou‐El‐Ela , S. H. , Prasse , K. W. , Farrell , R. I. , Carroll , R. W. , Wade , A. E. and Bunce , O. R.Effects of D, L‐2‐difluoromethylornithine and indomethacin on mammary tumor promotion in rats fed high n‐3 and/or n‐6 fat diets . Cancer Res. , 49 , 1434 – 1440 ( 1989. ). [PubMed] [Google Scholar]

Articles from Japanese Journal of Cancer Research : Gann are provided here courtesy of Wiley

RESOURCES