Abstract
Human T cell leukemia virus type II (HTLV‐II) is endemic in a number of native American populations and high rates of infection have also been demonstrated in intravenous drug abusers (IVDAs). Studies of virus isolates in the latter population have shown the existence of two closely related subtypes of the virus, HTLV‐IIa and HTLV‐IIb. To characterize the viruses present in native Americans, we analyzed by nucleotide sequence analysis the proviruses from the Wayu, an aboriginal population residing in Colombia, South America. The results showed HTLV‐IIb infection in this population, and also demonstrated remarkable conservation of sequence when compared to the proviruses in IVDAs.
Keywords: HTLV‐IIb, Wayu people, Aborigines (Colombia)
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REFERENCES
- 1).Rosenblatt , J. D. , Chen , I. S. Y. and Wachsman , W.Infection with HTLV‐I and HTLV‐II: evolving concepts . Semin. Hematol. , 25 , 230 – 246 ( 1988. ). [PubMed] [Google Scholar]
- 2).Hall , W. W. , Takahashi , H. , Zhu , S. W. and Ijichi , S.Human T‐cell leukemia virus, type II (HTLV‐II) . In “ Frontiers in Infectious Diseases; Focus on HIV ,” ed. Levy J. A. , Weiss R. A. and Neu H. C. , pp . 156 – 172 ( 1993. ). Churchill Livingstone Press; , London . [Google Scholar]
- 3).Moloney , E. M. , Biggar , R. J. and Neel , J. V.Endemic human T cell lymphotropic virus type II infection among isolated Brazilian Americans . J. Infect. Dis. , 166 , 100 – 107 ( 1992. ). [DOI] [PubMed] [Google Scholar]
- 4).Lal , R. B. , Povoa , M. and Lai , A. A.Seroprevalence of HTLV‐II in Paragaminos, State of Para, Brazil . J. AIDS , 5 , 634 – 636 ( 1992. ). [PubMed] [Google Scholar]
- 5).Lee , H. , Swanson , P. , Shorty , V. S. , Zack , J. A. , Rosenblatt , J. D. and Chen , I. S.High rate of HTLV‐II infection in seropositive i.v. drug abusers in New Orleans . Science , 244 , 471 – 475 ( 1989. ). [DOI] [PubMed] [Google Scholar]
- 6).Hall , W. W. , Takahashi , H. , Liu , C. , Kaplan , M. H. , Scheewind , O. , Ijichi , S. , Nagashima , K. and Gallo , R. C.Multiple isolates and characteristics of human T‐cell leukemia virus type II . J. Virol. , 66 , 2456 – 2463 ( 1992. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
- 7).Takahashi , H. , Zhu , S. W. , Ijichi , S. , Vahlne , A. , Suzuki , H. and Hall , W. W.Nucleotide sequence analysis of human T‐cell leukemia virus, type II (HTLV‐II) isolates . AIDS Res. Hum. Retoviruses , 9 , 721 – 732 ( 1993. ). [DOI] [PubMed] [Google Scholar]
- 8).Hjelle , B. , Zhu , S. W. , Ijichi , S. , Takahashi , H. and Hall , W. W.Endemic HTLV‐II infection in Southwestern U.S. Indians involves two prototype variants of virus . J. Infect. Dis. , in press . [DOI] [PubMed] [Google Scholar]
- 9).Marchuk , D. , Drumm , M. , Saulino , A. and Collins , F. S.Construction of T‐vectors, a rapid and general system for direct cloning of unmodified PCR products . Nucleic Acids Res. , 19 , 1154 ( 1990. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
- 10).Chung , C. T. , Miemela , S. L. and Miller , R. H.One‐step preparation of competent Escherichia coli: transformation and storage of bacterial cells in the same solution . Proc. Natl. Acad. Sci. USA , 86 , 2172 – 2175 ( 1989. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
- 11).Duenas‐Barajas , E. , Bernal , J. E. , Vaught , D. R. , Briceno , I. , Duran , C. , Yanagihara , R. and Gajdusek , C.Coexistence of human T‐lymphotropic virus types I and II among the Wayuu Indians from the Guajira region of Colombia . AIDS Res. Hum. Retroviruses , 8 , 1851 – 1855 ( 1992. ). [DOI] [PubMed] [Google Scholar]
- 12).Shimotohno , K. , Takahashi , Y. , Shimizu , N. , Gojobori , T. , Golde , D. W. , Chen , I. S. Y. , Miwa , M. and Sugimura , T.Complete nucleotide sequence of an infectious clone of human T‐cell leukemia virus type II: an open reading frame for the protease gene . Proc. Natl. Acad. Sci. USA , 82 , 3101 – 3105 ( 1985. ). [DOI] [PMC free article] [PubMed] [Google Scholar]