Abstract
We examined transforming growth factor‐β (TGF‐β) activity in cerebrospinal fluid of 39 patients with various brain tumors, and found it in 10 glioma cases that had lesions related to subarachnoid space or ventricle. In one glioma case, TGF‐β detected on admission disappeared after radiation and chemotherapy. We confirmed that five glioma cell lines produced TGF‐β, and that four of them produced active form of TGF‐β directly. The active form of TGF‐β was also identified from cerebrospinal fluid before the acidification treatment in two cases. The calculated contents were 110 ng/ml and 18 ng/ml. These results indicate that active form of TGF‐β is directly produced by tumor cells in patients with glioma, and may contribute to immunodeficiency of the host.
Keywords: TGF‐β, Cerebrospinal fluid, Glioma, Brain tumor
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REFERENCES
- 1. ) Massagué , J.The TGF‐β family of growth and differentiation factors . Cell , 49 , 437 – 438 ( 1987. ). [DOI] [PubMed] [Google Scholar]
- 2. ) Sporn , M. B. , Roberts , A. B. , Wakefield , L. M. and Assoian , R. K.Transforming growth factor‐β. Biological function and chemical structure . Science , 233 , 532 – 534 ( 1986. ). [DOI] [PubMed] [Google Scholar]
- 3. ) Sporn , M. B. , Roberts , A. B. , de Larco , J. E. , Wakefield , L. M. and de Crombrugghe , B.Some recent advances in the chemistry and biology of transforming growth factor‐beta . J. Cell Biol. , 105 , 1039 – 1045 ( 1987. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
- 4. ) Shirai , Y. , Kawata , S. , Ito , N. , Tamura , S. , Takaishi , K. , Kiso , S. , Tsushima , H. and Matsuzawa , Y.Elevated levels of plasma transforming growth factor‐β in patients with hepatocellular carcinoma . Jpn. J. Cancer Res. , 83 , 676 – 679 ( 1992. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
- 5. ) Tada , T. , Ohzeki , S. , Utsumi , K. , Takeuchi , H. , Muramatsu , M. , Li , X‐F. , Shimizu , J. , Fujiwara , H. and Hamaoka , T.Transforming growth factor‐β‐induced inhibition of T cell function. Susceptibility difference in T cells of various phenotypes and functions and its relevance to immunosuppression in the tumor‐bearing state . J. Immunol. , 146 , 1077 – 1082 ( 1991. ). [PubMed] [Google Scholar]
- 6. ) Cheifetz , S. , Weatherbee , J. A. , Tsang , M. L. S. , Anderson , J. K. , Mole , J. E. , Lucas , R. and Massaguè , J.The transforming growth factor‐β system, a complex pattern of cross reactive ligands and receptors . Cell , 48 , 409 – 415 ( 1987. ). [DOI] [PubMed] [Google Scholar]
- 7. ) de Larco , J. E. and Todaro , G. J.Growth factors from murine sarcoma virus‐transformed cells . Proc, Natl. Acad. Sci. USA , 75 , 4001 – 4005 ( 1978. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
- 8. ) Roberts , A. B. , Anzano , M. A. , Lamb , L. C. , Smith , J. M. and Sporn , M. B.New class of transforming growth factors potentiated by epidermal growth factor: isolation from non‐neoplastic tissues . Proc. Natl Acad, Sci. USA , 78 , 5339 – 5343 ( 1981. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
- 9. ) Bodmer , S. , Strommer , K. , Frei , K. , Siepl , C. , Tribolet , N. D. , Held , I. and Fontana , A.Immunosuppression and transforming growth factor‐β in glioblastoma. Preferential production of transforming growth factor‐β . J. Immunol. , 143 , 3222 – 3229 ( 1989. ). [PubMed] [Google Scholar]
- 10. ) de Martin , R. , Haendler , B. , Hofer‐Warbinek , R. , Gaugitsch , H. , Wrann , M. , Schlusener , H. , Seifert , J. M. , Bodmer , S. , Fontana , A. and Hofer , E.Complementary DNA for human glioblastoma‐derived T cell suppressor factor, a novel member of the transforming growth factor‐β gene family . EMBO J. , 6 , 3673 – 3677 ( 1987. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
- 11. ) Danielpour , D. , Dart , L. L. , Flanders , K. C. , Roberts , A. B. and Sporn , M. B.Immunodetection and quantitation of the two forms of transforming growth factor‐beta (TGF‐β1 and TGF‐β2) secreted by cells in culture . J. Cell Physiol. , 138 , 79 – 86 ( 1989. ). [DOI] [PubMed] [Google Scholar]
- 12. ) McPherson , J. M. , Sawamura , S. J. , Ogawa , Y. , Dineley , K. , Carrillo , P. and Piez , K. A.The growth inhibitor of African green monkey (BSC‐1) cells is transforming growth factors β1 and β2 . Biochemistry , 28 , 3442 – 3447 ( 1989. ). [DOI] [PubMed] [Google Scholar]
- 13. ) Wakefield , L. M. , Smith , D. M. , Flanders , K. C. and Sporn , M. B.Latent transforming growth factor‐β from human platelets . J. Biol. Chem. , 263 , 7646 – 7654 ( 1988. ). [PubMed] [Google Scholar]
- 14. ) O'Connor‐McCourt , M. D. and Wakefield , L. M.Latent transforming growth factor‐β in serum. A specific complex with α2‐macrogloblin . J. Biol. Chem. , 262 , 14090 – 14099 ( 1987. ). [PubMed] [Google Scholar]