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. 1993 May;84(5):532–537. doi: 10.1111/j.1349-7006.1993.tb00172.x

Molecular Cloning of 19p13 Breakpoint Region in Infantile Leukemia with t(11;19)(q23;p13) Translocation

Shinsuke Iida 1,2, Masao Seto 1,✉, Kazuhito Yamamoto 1, Hirokazu Komatsu 1,2, Yukihiro Akao 1,3, Shinpei Nakazawa 4, Yutaka Ariyoshi 2, Toshitada Takahashi 5, Ryuzo Ueda 1
PMCID: PMC5919173  PMID: 8320170

Abstract

We studied the breakpoint regions involved in t(11;19)(q23;p13) translocation associated with infantile leukemias. Southern blot analysis with the partial cDNA clone for the MLL gene at 11q23 which we had isolated previously detected gene rearrangements in all three cell lines and three leukemia samples from the patients with t(11;19) translocation, indicating that these breakpoints were clustered within the 8.5 kb BamHI germline fragment detected by the probe. To study the breakpoint region, a genomic library of one of the cell lines, KOCL‐33, was made. We have isolated the der(19) allele containing the breakpoint as well as the germline alleles at 19p13 and 11q23. Using the genomic probes on chromosome 19 near the breakpoint, Southern blot analysis was performed. The breakpoints at 19p13 of the two other cell lines and the three leukemia samples were not located within 36 kilobases of the KOCL‐33 breakpoint, although pulsed‐field gel electrophoresis showed that the breakpoints of all three cell lines were on the same NruI fragment of 230 kilobases. These results showed that the breakpoints at 19p13 were not clustered like those at 11q23 in t(11;19) translocation.

Keywords: Infantile leukemia, t(11;19)(q23;p13), MLL/ALL‐1, Translocation

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REFERENCES

  • 1. ) Abe , R. and Sandberg , A. A.Significance of abnormalities involving chromosomal segment 11q22–25 in acute leukemia . Cancer Genet. Cytogenet. , 13 , 121 – 127 ( 1984. ). [DOI] [PubMed] [Google Scholar]
  • 2. ) Kaneko , Y. , Maseki , N. , Takasaki , N. , Sakurai , M. , Hayashi , Y. , Nakazawa , S. , Mori , T. , Sakurai , M. , Takeda , T. , Shikano , T. and Hiyoshi , Y.Clinical and hematologic characteristics in acute leukemia with 11q23 translocations . Blood , 67 , 484 – 491 ( 1986. ). [PubMed] [Google Scholar]
  • 3. ) Raimondi , S. C. , Peiper , S. C. , Kitchingman , G. R. , Behm , F. G. , Williams , D. L. , Hancock , M. L. and Mirro , J.Childhood acute lymphoblastic leukemia with chromosomal breakpoints at 11q23 . Blood , 73 , 1627 – 1634 ( 1989. ). [PubMed] [Google Scholar]
  • 4. ) Iida , S. , Saito , M. , Okazaki , T. , Seto , M. , Yamamoto , K. , Akao , Y. , Ogura , M. , Suzuki , H. , Ariyoshi , Y. , Koike , K. , Nitta , M. , Takahashi , T. , Ueda , R. and Nakazawa , S.Phenotypic and genotypic characterization of 14 leukemia and lymphoma cell lines with 11q23 translocations . Leuk. Res. , 16 , 1155 – 1163 ( 1992. ). [DOI] [PubMed] [Google Scholar]
  • 5. ) Gibbons , B. , Katz , F. E. , Ganly , P. and Chessells , J. M.Infant acute lymphoblastic leukaemia with t(11;19) . Br. J. Haematol. , 74 , 264 – 269 ( 1990. ). [DOI] [PubMed] [Google Scholar]
  • 6. ) Akao , Y. , Seto , M. , Takahashi , T. , Saito , M. , Utsumi , K. R. , Nakazawa , S. and Ueda , R.Rearrangements on chromosome 11q23 in hematopoietic tumor‐associated t(11;14) and t(11;19) translocations . Cancer Res. , 51 , 6708 – 6711 ( 1991. ). [PubMed] [Google Scholar]
  • 7. ) Akao , Y. , Seto , M. , Yamamoto , K. , Iida , S. , Nakazawa , S. , Inazawa , J. , Abe , T. , Takahashi , T. and Ueda , R.The RCK gene associated with t(11;14) translocation is distinct from the MLL/ALL‐1 gene with t(4;11) and t(11;19) translocations . Cancer Res. , 52 , 6083 – 6087 ( 1992. ). [PubMed] [Google Scholar]
  • 8. ) Lu , D. and Yunis , J. J.Cloning, expression and localization of an RNA helicase gene from a human lymphoid cell line with chromosomal breakpoint 11q23.3 . Nucleic Acids Res. , 20 , 1967 – 1972 ( 1992. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 9. ) Rowley , J. D. , Diaz , M. O. , Espinosa , R. , III , Patel , Y. D. , Melle , E. V. , Ziemin , S. , Taillon‐Miller , P. , Lichter , P. , Evans , G. A. , Kersey , J. H. , Ward , D. C. , Domer , P. H. and LeBeau , M. M.Mapping chromosome band 11q23 in human acute leukemia with biotinylated probes: identification of 11q23 translocation breakpoints with a yeast artificial chromosome . Proc. Natl. Acad. Sci. USA , 87 , 9358 – 9362 ( 1990. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 10. ) van der Poel , Z. S. , McCabe , N. R. , Gill , H. J. , Espinosa , R. , III , Patel , Y. , Harden , A. , Rubinelli , P. , Smith , S. D. , LeBeau , M. M. , Rowley , J. D. and Diaz , M. O.Identification of a gene, MLL, that spans the breakpoint in 11q23 translocations associated with human leukemias . Proc. Natl. Acad. Sci. USA , 88 , 10735 – 10739 ( 1991. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 11. ) Djabali , M. , Selleri , L. , Parry , P. , Bower , M. , Young , B. D. and Evans , G. A.A trithorax‐like gene is interrupted by chromosome 11q23 translocations in acute leukaemias . Nature Genet. , 2 , 113 – 118 ( 1992. ). [DOI] [PubMed] [Google Scholar]
  • 12. ) Gu , Y. , Nakamura , T. , Prasad , A. R. , Canaani , O. , Cimino , G. , Croce , C. M. and Canaani , E.The t(4;11) chromosome translocation of human acute leukemias fuses the ALL‐1 gene, related to Drosophila trithorax, to the AF‐4 gene . Cell , 71 , 701 – 708 ( 1992. ). [DOI] [PubMed] [Google Scholar]
  • 13. ) Tkachuk , D. C. , Kohler , S. and Cleary , M. L.Involvement of a homolog of Drosophila trithorax by 11q23 chromosomal translocations in acute leukemias . Cell , 71 , 691 – 700 ( 1992. ). [DOI] [PubMed] [Google Scholar]
  • 14. ) McCabe , N. R. , Burnett , R. C. , Gill , H. J. , Thirman , M. J. , Mbangkollo , D. , Kipiniak , M. , van Melle , E. , van der Poel , Z. S. , Rowley , J. D. and Diaz , M. O.Cloning of cDNAs of the MLL gene that detect DNA rearrangements and altered RNA transcripts in human leukemic cells with 11q23 translocations . Proc. Natl. Acad. Sci. USA , 89 , 11794 – 11798 ( 1992. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 15. ) Cimino , G. , Moir , D. T. , Canaani , O. , Williams , K. , Crist , W. M. , Katzav , S. , Cannizzaro , L. , Lange , B. , Nowell , P. C. , Croce , C. M. and Canaani , E.Cloning of ALL‐1, the locus involved in leukaemias with the t(4;11)(q21;q23), t(9;11)(p22;q23), and t(11;19)(q23;p13) chromosome translocations . Cancer Res. , 51 , 6712 – 6714 ( 1991. ). [PubMed] [Google Scholar]
  • 16. ) Yamamoto , K. , Seto , M. , Akao , Y. , Iida , S. , Nakazawa , S. , Oshimura , M. , Takahashi , T. and Ueda , R.Gene rearrangement and truncated mRNA in cell lines with 11q23 translocation . Oncogene , 8 , 479 – 485 ( 1993. ). [PubMed] [Google Scholar]
  • 17. ) Seto , M. , Yamamoto , K. , Iida , S. , Akao , Y. , Utsumi , K. R. , Kubonishi , I. , Miyoshi , I. , Ohtsuki , T. , Yawata , Y. , Namba , M. , Motokura , T. , Arnold , A. , Takahashi , T. and Ueda , R.Gene rearrangement and overexpression of PRAD1 in lymphoid malignancy with t(11;14)(q13;q32) translocation . Oncogene , 7 , 1401 – 1406 ( 1992. ). [PubMed] [Google Scholar]
  • 18. ) Mitani , K. , Sato , Y. , Kobayashi , Y. , Shibazaki , Y. , Kasuga , M. , Inaba , T. , Hayashi , Y. , Miura , Y. , Miyazono , K. , Hirai , H. , Urabe , A. and Takaku , F.Heterogeneity in the breakpoints of chromosome 19 among acute leukemic patients with the t(11;19)(q23;p13) translocation . Am. J. Hematol. , 31 , 253 – 257 ( 1989. ). [DOI] [PubMed] [Google Scholar]
  • 19. ) Cimino , G. , Nakamura , T. , Gu , Y. , Canaani , O. , Prasad , R. , Crist , W. M. , Carroll , A. J. , Baer , M. , Bloomfield , C. D. , Nowell , P. C. , Croce , C. M. and Canaani , E.An altered 11‐kilobase transcript in leukemic cell lines with the t(4;11)(q21;q23) chromosome translocation . Cancer Res. , 52 , 3811 – 3813 ( 1992. ). [PubMed] [Google Scholar]
  • 20. ) Gu , Y. , Cimino , G. , Alder , H. , Nakamura , T. , Prasad , R. , Canaani , O. , Moir , D. T. , Jones , C. , Nowell , P. C. , Croce , C. M. and Canaani , E.The (4;11)(q21:q23) chromosome translocations in acute leukemias involve the VDJ recombinase . Proc. Natl. Acad. Sci. USA , 89 , 10464 – 10468 ( 1992. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 21. ) Bahkshi , A. , Wright , J. J. , Graninger , W. , Seto , M. , Owens , J. , Cossman , J. , Jensen , J. P. , Goldman , P. and Korsmeyer , S. J.Mechanism of the t(14;18) chromosomal translocation: structural analysis of both derivative 14 and 18 reciprocal partners . Proc. Natl. Acad. Sci. USA. , 84 , 2396 – 2400 ( 1987. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 22. ) Osada , H. , Seto , M. , Ueda , R. , Emi , N. , Takagi , N. , Obata , Y. , Suchi , T. and Takahashi , T.bcl‐2 gene rearrangement analysis in Japanese B cell lymphoma; novel bcl‐2 recombination with immunoglobulin κ chain gene . Jpn. J. Cancer Res. , 80 , 711 – 715 ( 1989. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 23. ) Seto , M. , Osada , H. , Ueda , R. , Ito , C. , Iwaki , O. , Oyama , A. , Suchi , T. and Takahashi , T.bcl‐2 translocation in Japanese B cell lymphoma: novel bcl‐2 translocation with immunoglobulin heavy chain diversity segment . Jpn. J. Cancer Res. , 82 , 65 – 71 ( 1991. ). [DOI] [PMC free article] [PubMed] [Google Scholar]

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