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Japanese Journal of Cancer Research : Gann logoLink to Japanese Journal of Cancer Research : Gann
. 1994 Nov;85(11):1117–1123. doi: 10.1111/j.1349-7006.1994.tb02916.x

Facilitation of Autonomous Phenotype Acquisition in Androgen‐dependent Shionogi Carcinoma 115 Cells by Transfection of Androgen‐induced Growth Factor Expression Vector

Yoshihiro Miyashita 1, Masafumi Koga 1,✉, Haruhiko Kouhara 1, Akira Tanaka 2, Tadamitsu Kishimoto 1, Bunzo Sato 1,†
PMCID: PMC5919376  PMID: 7829396

Abstract

Androgen‐induced growth factor (AIGF) is an autocrine growth factor for androgen‐dependent SC‐3 cells, which is induced by androgen stimuli. To elucidate the mechanism of the progression from hormone‐dependent to ‐independent tumor, we transfected an expression vector of cDNA encoding AIGF into SC‐3 cells and established a stable transfectant (Al) expressing AIGF. Al cells showed enhanced DNA synthesis. This enhanced DNA synthesis was blocked by exposing the cells to AIGF autisense oligonucleotides, heparin, or suramin, indicating that enforced AIGF expression is responsible for the increase in DNA synthesis. However, Al cells did not grow in serum‐free medium unless stimulated with androgen. Recloning from Al cells in semi‐solid agar supplemented with fetal calf serum but without androgen quickly generated an autonomous subline that was able to grow rapidly in the serum‐free medium irrespective of androgen stimulus. Mock‐transfected SC‐3 cells failed to form any colony under identical conditions. These results suggest that stable expression of AIGF alone is not sufficient for, but facilitates the conversion of SC‐3 cells from androgen‐dependent to ‐independent phenotype.

Keywords: Androgen‐dependent cancer, Growth factor, Stable transfectant, Androgen dependency

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REFERENCES

  • 1. ) Haggins , C. and Bergenstal , D. M.Inhibition of human mammary and prostatic cancers by adrenalectomy . Cancer Res. , 12 , 134 – 141 ( 1952. ). [PubMed] [Google Scholar]
  • 2. ) Jensen , E. V. , Polly , T. Z. , Smith , S. , Block , G. E. , Ferguson , D. J. and DeSombre , E. R.Prediction of hormone dependency in human breast cancer . In “ Estrogen Receptors in Human Breast Cancer, ” ed , McGuire W. L. , Carbone P. P. and Vollmer E. P. , pp. 37 – 54 ( 1975. ). Raven Press; , New York . [Google Scholar]
  • 3. ) Evans , R. M.The steroid and thyroid hormone receptor superfamily . Science , 240 , 889 – 895 ( 1988. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 4. ) Beato , M.Gene regulation by steroid hormones: review . Cell , 56 , 335 – 344 ( 1989. ). [DOI] [PubMed] [Google Scholar]
  • 5. ) Cole , M. P. , Jones , C. T. A. and Todd , I. D. H.A new antiestrogenic agent in late breast cancer. An early clinical appraisal of ICI 46474 . Br. J. Cancer , 25 , 270 – 275 ( 1971. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 6. ) Vogelzang , N. J. and Rennealey , G. T.Recent developments in endocrine treatment of prostate cancer . Cancer , 70 ( Suppl. ), 966 – 975 ( 1992. ). [PubMed] [Google Scholar]
  • 7. ) Furr , B. J. A. and Jordan , V. C.The pharmacology and clinical uses of tamoxifen . Pharmacol. Ther. , 25 , 127 – 205 ( 1984. ). [DOI] [PubMed] [Google Scholar]
  • 8. ) Sneller , Z. W. , Hop , W. C. J. , Carpentier , P. J. and Schröder , F. H.Prognosis and prostatic volume changes during endocrine management of prostate cancer: a longitudinal study . J. Urol. , 147 , 962 – 966 ( 1992. ). [DOI] [PubMed] [Google Scholar]
  • 9. ) Dickson , R. B. , Huff , K. K. , Spencer , E. M. and Lippman , M. E.Induction of epidermal growth factor‐related pep‐tides by 17 β‐estradioI in MCF‐7 human breast cancer cell . Endocrinology , 118 , 138 – 145 ( 1986. ). [DOI] [PubMed] [Google Scholar]
  • 10. ) Lippman , M. E. , Dickson , R. B. , Bates , S. , Knabbe , C. , Huff , K. , Swain , S. , McManaway , M. , Brozert , D. and Kasid , A.Autocrine and paracrine growth regulation of human breast cancer . Breast Cancer Res. Treat. , 7 , 59 – 70 ( 1986. ). [DOI] [PubMed] [Google Scholar]
  • 11. ) Dickson , R. B. , McManaway , M. E. , and Lippman , M. E.Estrogen‐induced factors of breast cancer cells partially replace estrogen to promote tumor growth . Science , 232 , 1540 – 1543 ( 1987. ). [DOI] [PubMed] [Google Scholar]
  • 12. ) Clarke , R. , Briinner , N. , Katz , D. , Glanz , P. , Dickson , R. B. , Lippman , M. E. and Kern , F. G.The effects of a constitutive expression of transforming growth factor‐α on the growth of MCF‐7 human breast cancer cells in vivo and in vitro . Mol Endocrinol. , 3 , 372 – 380 ( 1989. ). [DOI] [PubMed] [Google Scholar]
  • 13. ) Arteaga , C. L. , Coronado , E. and Osborne , C. K.Blockade of the epidermal growth factor receptor inhibits transforming growth factor‐α‐induced but not estrogen‐induced growth of hormone‐dependent human breast cancer . Mol. Endocrinol , 2 , 1064 – 1069 ( 1988. ). [DOI] [PubMed] [Google Scholar]
  • 14. ) Noguchi , S. , Nishizawa , Y. , Nakamura , N. , Uchida , N.Yamaguchi , K. , Sato , B. , Kitamura , Y. and Matsumoto , K.Growth stimulating effect of pharmacological doses of estrogen on androgen‐dependent Shionogi carcinoma 115 in vivo but not in cell culture . Cancer Res. , 47 , 263 – 268 ( 1987. ). [PubMed] [Google Scholar]
  • 15. ) Tanaka , A. , Miyamoto , K. , Minamino , N. , Takeda , M. , Sato , B. , Matsuo , H. and Matsumoto , K.Cloning and characterization of an androgen‐induced growth factor essential for the androgen‐dependent growth of mouse mammary carcinoma cells . Proc. Natl. Acad. Sci. USA , 89 , 8928 – 8932 ( 1992. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 16. ) Kouhara , H. , Koga , M. , Kasayama , S. , Tanaka , A. , Kishimoto , T. and Sato , B.Transforming activity of a newly cloned androgen‐induced growth factor . Oncogene , 9 , 455 – 462 ( 1994. ). [PubMed] [Google Scholar]
  • 17. ) Chen , C. and Okayama , H.High‐efficiency transformation of mammalian cells by plasmid DNA . Mol. Cell, Biol , 7 , 2745 – 2752 ( 1987. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 18. ) Sasada , R. , Kurokawa , T. , Iwane , M. and Igarashi , K.Transformation of mouse BALB/c 3T3 cells with human basic fibroblast growth factor cDNA . Mol. Cell. Biol. , 8 , 588 – 594 ( 1988. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 19. ) Yamanishi , H. , Nonomura , N. , Tanaka , A. , Nishizawa , Y. , Terada , N. , Matsumoto , K. and Sato , B.Proliferation of Shionogi carcinoma 115 cells by glucocorticoid‐induced autocrine heparin‐binding growth factor(s) in serum‐free medium . Cancer Res. , 51 , 3006 – 3010 ( 1991. ). [PubMed] [Google Scholar]
  • 20. ) Saito , H. , Kasayama , S. , Kouhara , H. , Matsumoto , K. and Sato , B.Up‐regulation of fibroblast growth factor (FGF) receptor mRNA levels by basic FGF or testosterone in androgen‐sensitive mouse mammary tumor cells . Biochem. Biophys. Res. Common. , 174 , 136 – 141 ( 1991. ). [DOI] [PubMed] [Google Scholar]
  • 21. ) Kasayama , S. , Saito , H. , Kouhara , H. , Sumitani , S. and Sato , B.Suramin interrupts androgen‐inducible autocrine loop involving heparin binding growth factor in mouse mammary cancer (Shionogi carcinoma 115) cells . J. Cell Physiol. , 154 , 254 – 261 ( 1993. ). [DOI] [PubMed] [Google Scholar]
  • 22. ) Kasayama , S. , Sumitani , S. , Tanaka , A. , Yamanishi , H. , Nakamura , N. , Matsumoto , K. and Sato , B.Heparin inhibits autocrine stimulation but not fibroblast growth factor stimulation of cell proliferation of androgen‐responsive Shionogi carcinoma 115 . J. Cell Physiol. , 148 , 260 – 266 ( 1991. ). [DOI] [PubMed] [Google Scholar]
  • 23. ) Dabre , P. D. and King , R. J. B.Differential effects of steroid hormones on parameters of cell growth . Cancer Res. , 47 , 2937 – 2944 ( 1987. ). [PubMed] [Google Scholar]
  • 24. ) Sato , N. , Watabe , Y. , Suzuki , H. and Shimazaki , J.Progression of androgen‐sensitive mouse tumor (Shionogi carcinoma 115) to androgen‐insensitive tumor after long‐term removal of testosterone . Jpn. J. Cancer Res. , 84 , 1300 – 1308 ( 1993. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 25. ) Sumitani , S. , Kasayama , S. , Hirose , T. , Matsumoto , K. and Sato , B.Effects of thyroid hormone on androgen‐ or basic fibroblast growth factor‐induced proliferation of Shionogi carcinoma 115 mouse mammary carcinoma cells in serum‐free culture . Cancer Res. , 51 , 4323 – 4327 ( 1991. ). [PubMed] [Google Scholar]
  • 26. ) Nawata , H. , Bronzert , D. and Lippman , M. E.Isolation and characterization of a tamoxifen‐resistant cell line derived from MCF‐7 human breast cancer cells . J. Biol. Chem. , 256 , 5016 – 5021 ( 1981. ). [PubMed] [Google Scholar]

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