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Japanese Journal of Cancer Research : Gann logoLink to Japanese Journal of Cancer Research : Gann
. 1994 Sep;85(9):887–891. doi: 10.1111/j.1349-7006.1994.tb02964.x

Expression of Tumor‐associated Glycoantigen, Sialyl Lewisa, in Human Head and Neck Squamous Cell Carcinoma and Its Application to Tumor Immunotherapy

Koji Makino 1, Tetsuro Ogata 1, Hirosato Miyake 1, Sonoko Habu 2, Takashi Nishimura 2,✉
PMCID: PMC5919591  PMID: 7961115

Abstract

The glycoantigen sialyl Lewisa (sLea) is widely expressed on a variety of gastrointestinal tumor cells. Here, we immunohistochemically demonstrated the expression of sLea antigen in 54% (7 out of 13) of human head and neck squamous cell carcinoma (H‐NSCG) samples. Frequent expression of sLea antigen was also demonstrated on a variety of H‐NSCC cell lines using flow cytometry. Both CD4+ and CD8+ T cells, which were activated with immobilized OKT3 monoclonal antibody plus inter‐leukin‐2, showed augmented cytotoxicity against sLea‐positive H‐NSCC, including autologous tumor cells, on targeting with anti‐CD3 x anti‐sLea bispccific antibody, suggesting that sLea antigen is a good target molecule for bispecific antibody‐dependent adoptive tumor immunotherapy of human head and neck cancer.

Keywords: Sialyl Lewisa, Head and neck squamous cell carcinoma, Immunotherapy

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