Abstract
Gangliosides of liposarcoma, lipoma and lipids from omental tissues were analyzed. By immunostaining after thin layer chromatography, gangliosides of liposarcoma were identified as GM3, sialyl‐paragloboside and GD3, whereas those of lipoma were GM3 and GD3, and those of fat in omental tissues were GM3, GD1a, GD3 and some unknown ones. Expression of sialylparagloboside is thought to be very rare.
Keywords: Ganglioside, Liposarcoma, Lipoma, Adipose tissue, Monoclonal antibody
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REFERENCES
- 1).Kohler , G. and Milstein , C.Continuous cultures of fused cells secreting antibody of predefined specificity . Nature. , 256 , 495 – 497 ( 1975. ). [DOI] [PubMed] [Google Scholar]
- 2).Hakomori , S. and Kannagi , R.Glycosphingolipids as tumor‐associated and differentiation markers . J. Natl. Cancer Inst. , 71 , 231 – 251 ( 1983. ). [PubMed] [Google Scholar]
- 3).Svennerhlom , L.The gangliosides . J. Lipid Res. , 5 , 145 – 155 ( 1964. ). [PubMed] [Google Scholar]
- 4).Houghton , A. N. , Mintzer , D. , Cordon‐Cardo , C. , Welt , S. , Fliegel , B. , Vadhan , S. , Carswell , E. , Melamed , M. R. , Oettgen , H. F. and Old , L. J.Mouse monoclonal antibody detecting GD3 ganglioside: a phase I trial in patients with malignant melanoma . Proc. Natl. Acad. Sci. USA , 82 , 1242 – 1246 ( 1985. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
- 5).Mujoo , K. , Cheresh , D. A. , Yang , H. M. and Reisfeld , R. A.Disialoganglioside GD2 on human neuroblastoma cells: target antigen for monoclonal antibody‐mediated cytolysis and suppression of tumor growth . Cancer Res. , 47 , 1098 – 1104 ( 1987. ). [PubMed] [Google Scholar]
- 6).Huang , L. C. , Civin , C. I. , Magnani , J. L. , Shaper , J. H. and Ginsburg , V.My‐1, the human myeloid‐specific antigen detected by mouse monoclonal antibodies, is a sugar sequence found in lacto‐N‐fucopentaose III . Blood , 61 , 1020 – 1023 ( 1983. ). [PubMed] [Google Scholar]
- 7).Brockhause , M. , Magnani , J. L. , Herlyn , M. , Blaszczyk , M. and Ginsburg , V.Monoclonal antibodies directed against the sugar sequence of lacto‐N‐fucopentaose III obtained from mice immunized with human tumors . Fed. Proc. , 41 , 897 ( 1982. ). [DOI] [PubMed] [Google Scholar]
- 8).Hiraiwa , N. , Iida , N. , Ishizuka , I. , Itai , S. , Shigeta , K. , Kannagi , R. , Fukuda , Y. and Imura , H.Monoclonal antibodies directed to a disulfated glycosphingolipid, SBla (GgOse4Cer‐II3IV3‐bis‐sulfate), associated with human hepatocellular carcinoma . Cancer Res. , 48 , 6769 – 6774 ( 1988. ). [PubMed] [Google Scholar]
- 9).Falk , K. ‐E. , Karlsson , K.‐A. , Larson , G. , Thurin , J. , Blaszczyk , M. , Steplewski , Z. and Koprowski , H.Mass spectrometry of a human tumor glycolipid antigen defined by mouse monoclonal antibody NS‐19–9 . Biochem. Biophys. Res. Commun. , 110 , 383 – 391 ( 1983. ). [DOI] [PubMed] [Google Scholar]
- 10).Magnani , J. L. , Nilson , B. , Brockhouse , M. , Zopf , D. , Steplewski , Z. , Koprowski , H. and Ginsburg , V.A monoclonal antibody‐defined antigen associated with gastrointestinal cancer is a ganglioside containing sialylated lacto‐N‐fucopentaose II . J. Biol. Chem. , 257 , 14365 – 14369 ( 1982. ). [PubMed] [Google Scholar]
- 11).Lloid , K. O. , Larson , G. , Stromberg , N. , Thurin , J. and Karlsson , K.‐A.Mouse monoclonal antibody F‐3 recognizes the difucosyl type‐2 blood group structure . Immunogenetics , 17 , 537 – 541 ( 1983. ). [DOI] [PubMed] [Google Scholar]
- 12).Kannagi , R. , Nudelman , E. , Levery , S. and Hakomori , S.A series of human erythrocyte glycosphingolipids reacting to the monoclonal antibody directed to a developmentally regulated antigen, SSEA‐1 . J. Biol. Chem. , 257 , 14865 – 14874 ( 1982. ). [PubMed] [Google Scholar]
- 13).Hakomori , S.Aberrant glycosylation in cancer cell membranes as focused on glycolipids: overview and prospectives . Cancer Res. , 45 , 2405 – 2414 ( 1985. ). [PubMed] [Google Scholar]
- 14).Kinne , D. W. , Chu , F. C. M. , Huvos , A. G. , Yagoda , A. and Fortner , J. G.Treatment of primary and recurrent retroperitoneal liposarcoma . Cancer , 31 , 51 – 64 ( 1973. ). [DOI] [PubMed] [Google Scholar]
- 15).Bek , V.Primary, retroperitoneal tumours . Neoplasma , 17 , 253 – 263 ( 1970. ). [PubMed] [Google Scholar]
- 16).Ledeen , R. W. , Yu , R. K. and Eng , L. F.Gangliosides of human myelin: sialosylgalactosylceramide (G7) as a major component . Neurochem. , 21 , 829 – 839 ( 1973. ). [DOI] [PubMed] [Google Scholar]
- 17).Kotani , M. , Ozawa , H. , Kawashima , I. , Ando , S. and Tai , T.Generation of one set of monoclonal antibodies specific for a‐pathway ganglio‐series gangliosides . Bio-chim. Biophys. Acta , 1117 , 97 – 103 ( 1992. ). [DOI] [PubMed] [Google Scholar]
- 18).Ozawa , H. , Kotani , M. , Kawashima , I. and Tadashi , T.Generation of one set of monoclonal antibodies specific for b‐pathway ganglio‐series gangliosides . Biochim. Biophys. Acta , 1123 , 184 – 190 ( 1992. ). [DOI] [PubMed] [Google Scholar]
- 19).Li , S. , Kundu , S. K. , Degasperi , R. and Li , Y.Accumulation of globotriaosylceramide in a case of leiomyosarcoma . Biochem. J. , 240 , 925 – 927 ( 1986. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
- 20).Fukushima , K. , Hirota , M. , Terasaki , P. I. , Wakisaka , A. , Togashi , H. , Chia , D. , Suyama , N. , Fukushi , Y. , Nudelman , E. and Hakomori , S.Characterization of sialosylated Lewisx as a new tumor‐associated antigen . Cancer Res. , 44 , 5279 – 5285 ( 1984. ). [PubMed] [Google Scholar]
- 21).Steffens , T. A. , Bajorin , D. F. and Houghton , A. N.Immunotherapy with monoclonal antibodies in metastatic melanoma . World J. Surg. , 16 , 261 – 269 ( 1992. ). [DOI] [PubMed] [Google Scholar]
