Abstract
Because of the potential significance of colonic bacteria in colon carcinogenesis, we investigated the effect of pectin of different types on fecal bacterial enzymes (β‐glucuronidase, β‐glucosidase and tryptophanase) at various periods of time after feeding rats with pectin‐containing diets during azoxymethane‐induced colon carcinogenesis. The diet supplemented with 20% apple pectin or 20% citrus pectin decreased the multiplicity of colon tumors, and the number of tumors was significantly decreased in the group fed apple pectin. The incidence of colon tumors in the apple pectin group was lower than that in the control group. The mean tumor size was similar among the three groups. Apple pectin feeding decreased fecal β‐glucosidase and tryptophanase levels. Furthermore, a significant decrease in the activity of β‐glucuronidase was observed in the apple pectin group during the initiation phase. These findings suggest that the protective effect of pectin on colon carcinogenesis may be dependent on the type of pectin and be related to the decrease of β‐glucuronidase activity in the initiation stage of carcinogenesis.
Keywords: Dietary fiber, Colon cancer prevention, Apple pectin, Azoxymethane, Fecal bacterial enzymes
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REFERENCES
- 1. ) Burkitt , D. P.Epidemiology of cancer of the colon and rectum . Cancer , 28 , 3 – 13 ( 1971. ). [DOI] [PubMed] [Google Scholar]
- 2. ) Watanabe , K. , Reddy , B. S. , Weisburger , J. H. and Kritchevsky , D.Effect of dietary alfalfa, pectin, and wheat bran on azoxymethane or methylnitrosourea‐induced colon carcinogenesis in F344 rats . J. Natl. Cancer Inst. , 63 , 141 – 145 ( 1979. ). [PubMed] [Google Scholar]
- 3. ) Bauer , H. G. , Asp , N. G. , Dahlqvist , A. , Fredlund , P. E. , Nyman , M. and Oste , R.Effects of two kinds of pectin and guar gum on 1,2‐dimethylhydrazine initiation of colon tumors and fecal β‐glucuronidase activity in the rat . Cancer Res. , 41 , 2518 – 2523 ( 1981. ). [PubMed] [Google Scholar]
- 4. ) Jacobs , L. R. and Lupton , J. R.Relationship between colonic luminal pH, cell proliferation, and colon carcinogenesis in 1,2‐dimethylhydrazine treated rats fed high fiber diets . Cancer Res. , 46 , 1727 – 1734 ( 1986. ). [PubMed] [Google Scholar]
- 5. ) Masaki , K.Effect of dietary fibers on colon carcinogenesis induced by 1,2‐dimethylhydrazine in rat . J. Wakayama Med. Soc. , 44 , 351 – 364 ( 1993. ) ( in Japanese ). [Google Scholar]
- 6. ) Freeman , H. J.Effects of differing purified cellulose, pectin, and hemicellulose fiber diets on fecal enzymes in 1,2‐dimethylhydrazine‐induced rat colon carcinogenesis . Cancer Res. , 46 , 5529 – 5532 ( 1986. ). [PubMed] [Google Scholar]
- 7. ) Bauer , H. G. , Asp , N. G. , Oste , R. , Dahlqvist , A. and Fredlund , P. E.Effect of dietary fiber on the introduction of colorectal tumors and fecal β‐glucuronidase activity in the rat . Cancer Res. , 39 , 3752 – 3756 ( 1979. ). [PubMed] [Google Scholar]
- 8. ) Freeman , H. J. , Spiller , G. A. and Kim , Y. S.A double‐blind study on the effects of differing purified cellulose and pectin fiber diets on 1,2‐dimethylhydrazine‐induced rat colonic neoplasia . Cancer Res. , 40 , 2661 – 2665 ( 1980. ). [PubMed] [Google Scholar]
- 9. ) Heitman , D. W. , Hardman , W. E. and Cameron , I. L.Dietary supplementation with pectin and guar gum on 1,2‐dimethylhydrazine‐induced colon carcinogenesis in rats . Carcinogenesis , 13 , 815 – 818 ( 1992. ). [DOI] [PubMed] [Google Scholar]
- 10. ) Tazawa , K. , Ookami , H. , Yamashita , I. , Shimizu , T. , Fujimaki , M. , Murai , K. , Kobashi , K. and Honda , T.Effect of apple pectin on azoxymethane‐induced colon carcinogenesis—fecal enzyme activities and prostaglandin E2 level in colonic mucosa , In “ Recent Advantage in Management of Digestive Cancers ,” ed. Takahashi T. , pp. 471 – 473 ( 1993. ). Springer‐Verlag; , Tokyo . [Google Scholar]
- 11. ) Ookami , H. , Tazawa , K. , Okamoto , M. , Yamashita , I. , Masuyama , K. , Shimizu , T. , Yamamoto , K. , Katsuyama , S. , Takemori , S. , Arai , H. , Sakamoto , T. , Yamashita , Y. , Maeda , M. , Honda , T. and Fujimaki , M.Effect of apple pectin on azoxymethane‐induced colon carcinogenesis (first report); prostaglandin E2 level in colonic mucosa and portal vein . J. Jpn. Res. Soc. Gastroenterol. Carcinog. , 5 , 91 – 94 ( 1993. ) ( in Japanese ). [Google Scholar]
- 12. ) Tazawa , K.Bacteriostatical properties of skin barriers . Proc. 7th Bienn. Congr. World Council of Enterostomal Therapists , 37 – 41 ( 1988. ).
- 13. ) Simon , G. L. and Gorbach , S. L.Intestinal flora in health and disease . Gastroenterology , 86 , 174 – 193 ( 1984. ). [PubMed] [Google Scholar]
- 14. ) Goldin , B. R. and Gorbach , S. L.The relationship between diet and rat fecal bacterial enzymes implicated in colon cancer . J. Natl. Cancer Inst. , 57 , 371 – 375 ( 1978. ). [DOI] [PubMed] [Google Scholar]
- 15. ) Prizont , R. and Konisberg , N.Identification of bacterial glycosidases in rat fecal contents . Dig. Dis. Sci. , 26 , 773 – 777 ( 1981. ). [DOI] [PubMed] [Google Scholar]
- 16. ) Scheline , R. R.Metabolism of foreign compounds by gastrointestinal microorganisms . Pharmacol. Rev. , 25 , 451 – 523 ( 1973. ). [PubMed] [Google Scholar]
- 17. ) Akao , T. , Akao , T. and Kobashi , K.Glycyrrhizin β‐D‐glucuronidase of Eubacterium sp. from human intestinal flora . Chem. Pharm. Bull. , 35 , 705 – 710 ( 1987. ). [DOI] [PubMed] [Google Scholar]
- 18. ) Demoss , R. D. and Moser , K.Tryptophanase in diverse bacterial species . J. Bacteriol. , 98 , 167 – 171 ( 1969. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
- 19. ) Williams , R. T.Toxicologic implications of biotransformation by intestinal microflora . Toxicol. Appl. Pharmacol. , 23 , 769 – 781 ( 1972. ). [DOI] [PubMed] [Google Scholar]
- 20. ) Chipman , J. K.Biles as a source of potential reactive metabolites . Toxicology , 25 , 99 – 111 ( 1982. ). [DOI] [PubMed] [Google Scholar]
- 21. ) Laqueur , G. L.Carcinogenic effects of cycad meal and cycasin, methylazoxymethanol glycoside, in rats and effects of cycasin in germfree rats . Fed. Proc. , 23 , 1386 – 1388 ( 1964. ). [PubMed] [Google Scholar]
- 22. ) Laqueur , G. L.Contribution of intestinal macroflora and microflora to carcinogenesis , In “ Carcinoma of the Colon and Antecedent Epithelium ,” ed. Burdette W. J. , pp. 305 – 313 ( 1970. ). Charles C. Thomas Publisher; , Springfield , IL . [Google Scholar]
- 23. ) Laqueur , G. L. and Spatz , M.Oncogenicity of cycasin and methylazoxymethanol . Gann Monogr. Cancer Res. , 17 , 189 – 204 ( 1975. ). [Google Scholar]
- 24. ) Narisawa , T. and Nakano , H.Carcinoma of the large intestine of rats induced by rectal infusion of methylazoxymethanol . Gann , 64 , 93 – 95 ( 1973. ). [PubMed] [Google Scholar]
- 25. ) Prizont , R.Influence of high dietary cellulose on fecal glucosidases in experimental rat colon carcinogenesis . Cancer Res. , 44 , 557 – 561 ( 1984. ). [PubMed] [Google Scholar]
- 26. ) Chung , K. T. , Fulk , G. E. and Slein , M. W.Tryptophanase of fecal flora as a possible factor in the etiology of colon cancer . J. Natl. Cancer Inst. , 54 , 1073 – 1078 ( 1975. ). [DOI] [PubMed] [Google Scholar]
- 27. ) Weisburger , J. H. , Grantham , P. H. , Horton , R. E. and Weisburger , E. K.Metabolism of the carcinogen N‐hydroxy‐N‐2‐fluorenylacetamide in germfree rats . Biochem. Pharmacol. , 19 , 151 – 162 ( 1970. ). [DOI] [PubMed] [Google Scholar]
- 28. ) Weisburger , J. H.Colon carcinogens: their metabolism and mode of action . Cancer , 28 , 60 – 70 ( 1971. ). [DOI] [PubMed] [Google Scholar]
- 29. ) Weisburger , J. H.Chemical carcinogens and their mode of action in colonic neoplasma . Dis. Colon Rectum , 16 , 431 – 437 ( 1973. ). [DOI] [PubMed] [Google Scholar]
- 30. ) Takada , H. , Hirooka , T. , Hiramatsu , Y. and Yamamoto , M.Effect of β‐glucuronidase inhibitor on azoxymethane‐induced colonic carcinogenesis in rats . Cancer Res. , 42 , 331 – 334 ( 1982. ). [PubMed] [Google Scholar]