Abstract
In Southern blot analysis using Mycoplasma16S ribosomal DNA (rDNA) as a probe, positive signals were detected in DNA samples from surgical specimens of gastric cancers. The DNA that hybridized to Mycoplasma 16S rDNA was eluted from the gel, cloned and sequenced. The cloned sequence was identical to 16S rDNA of Streptococcus anginosus. In Southern blot analysis with the S. anginosus 16S rDNA fragment as a new probe, positive signals were detected in 9 (20%) out of 43 cases of gastric cancer.
Keywords: Gastric cancer, Streptococcus, Mycoplasma
Full Text
The Full Text of this article is available as a PDF (269.0 KB).
REFERENCES
- 1. ) Katoh , M. and Terada , M.Oncogene and tumor suppressor genes . In “ Gastric Cancer ,” ed. Nishi M. , Ichikawa H. , Nakajima T. , Maruyama K. and Tahara E. , pp. 196 – 208 ( 1993. ). Springer‐Verlag; , Tokyo . [Google Scholar]
- 2. ) Correa , P. , Fox , J. , Fontham , E. , Ruiz , B. , Lin , Y. P. , Zavala , D. , Taylor , N. , Mackinly , D. , de Lima , E. , Portilla , H. and Zarama , G.Helicobactor pylori and gastric carcinoma. Serum antibody prevalence in populations with contrasting cancer risks . Cancer , 66 , 2569 – 2574 ( 1990. ). [DOI] [PubMed] [Google Scholar]
- 3. ) Forman , D. , Sitas , F. , Newell , D. G. , Stacey , A. R. , Boreham , J. , Peto , R. , Campbell , T. C. , Li , J. and Chen , J.Geographic association of Helicobacter pylori antibody prevalence and gastric cancer mortality in rural China . Int. J. Cancer , 46 , 608 – 611 ( 1990. ). [DOI] [PubMed] [Google Scholar]
- 4. ) Forman , D.Helicobacter pylori infection: a novel risk factor in the etiology of gastric cancer (editorial) . J. Natl. Cancer Inst. , 83 , 1702 – 1703 ( 1991. ). [DOI] [PubMed] [Google Scholar]
- 5. ) Nomura , A. , Stemmermann , G. N. , Chyou , P. H. , Kato , I. , Perz , P. G. and Blaser , M. J.Helicobacter pylori infection and gastric carcinoma among Japanese Americans in Hawaii . N. Engl. J. Med. , 325 , 1132 – 1136 ( 1991. ). [DOI] [PubMed] [Google Scholar]
- 6. ) Parsonnet , J. , Friedman , G. D. , Vandersteen , D. P. , Chang , Y. , Vogelman , J. H. , Orentreich , N. and Sibley , R. K.Helicobacter pylori infection and the risk of gastric carcinoma . N. Engl. J. Med. , 325 , 1127 – 1131 ( 1991. ). [DOI] [PubMed] [Google Scholar]
- 7. ) Parsonnet , J. , Vandersteen , D. , Goates , J. , Sibley , R. K. , Pritikin , J. and Chang , Y.Helicobacter pylori infection in intestinal‐ and diffuse‐type gastric adenocarcinomas . J. Natl. Cancer Inst. , 83 , 640 – 643 ( 1991. ). [DOI] [PubMed] [Google Scholar]
- 8. ) Talley , N. J. , Zinsmeister , A. R. , Weaver , A. , DiMagno , E. P. , Carpenter , H. A. , Perez , P. G. and Blaser , M. J.Gastric adenocarcinoma and Helicobacter pylori infection . J. Natl. Cancer Inst. , 83 , 1734 – 1739 ( 1991. ). [DOI] [PubMed] [Google Scholar]
- 9. ) Sipponen , P. , Kosunen , T. U. , Valle , J. , Riihelar , M. and Seppala , K.Helicobacter pylori infection and chronic gastritis in gastric cancer . J. Clin. Pathol. , 45 , 319 – 323 ( 1992. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
- 10. ) Fukuda , H. , Saito , D. , Hayashi , S. , Hisai , H. , Ono , H. , Yoshida , S. , Oguro , Y. , Noda , T. , Sato , T. , Katoh , M. , Terada , M. and Sugimura , T.Helicobacter pylori infection, serum pepsinogen level and gastric cancer: a case‐control study in Japan . Jpn. J. Cancer Res. , 86 , 64 – 71 ( 1995. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
- 11. ) Sasaki , H. , Nomura , S. , Akiyama , N. , Takahashi , A. , Sugimura , T. , Oishi , M. and Terada , M.Highly efficient method for obtaining a subtracted genomic DNA library by the modified in‐gel competitive reassociation method . Cancer Res. , 54 , 5821 – 5823 ( 1994. ). [PubMed] [Google Scholar]
- 12. ) Uemori , T. , Asada , K. , Kato , I. and Harasawa , R.Amplification of the 16S‐23S spacer region in rRNA operons of mycoplasmas by the polymerase chain reaction . Syst. Appl. Microbiol. , 15 , 181 – 186 ( 1992. ). [Google Scholar]
- 13. ) Correa , P.Human gastric carcinogenesis: a multistep and multifactorial process. First American Cancer Society Award Lecture on Cancer Epidemiology and prevention . Cancer Res. , 52 , 6735 – 6740 ( 1992. ). [PubMed] [Google Scholar]
- 14. ) Parkin , M. D. , Stjernsward , J. and Muir , C.Estimates of the worldwide frequency of twelve major cancers . Int. J. Cancer. , 41 , 184 – 197 ( 1988. ). [DOI] [PubMed] [Google Scholar]
- 15. ) Palli , D. , Decarli , A. , Cipriani , F. , Forman , D. , Amadori , D. , Avelli , C. , Giacosa , A. , Manca , P. , Russo , A. , Salkeld , R. M. , Samloff , I. M. , Fraumoni , J. F. , Jr. , Blot , W. J. and Buiatti , E.Plasma pepsinogens, nutrients, and diet in areas of Italy at varying gastric cancer risk . Cancer Epidemiol. Bsomarkers Prev. , 1 , 45 – 50 ( 1991. ). [PubMed] [Google Scholar]
- 16. ) Tsugane , S. , Kabuto , M. , Imai , H. , Gey , F. , Tei , Y. , Hanaoka , T. , Sugano , K. and Watanabe , S.Helicobacter pylori, dietary factors, and atrophic gastritis in five Japanese populations with different gastric cancer mortality . Cancer Causes Control , 4 , 297 – 305 ( 1993. ). [DOI] [PubMed] [Google Scholar]
- 17. ) Ruoff , K. L.Streptococcus anginosus: the unrecognized pathogen . Clin. Microbiol. Rev. , 1 , 102 ( 1988. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
- 18. ) Fisher , L. E. and Russel , R. R.The isolation and characterization of milleri group streptococci from dental periapical abscesses . J. Dent. Res. , 72 , 1191 – 1193 ( 1993. ). [DOI] [PubMed] [Google Scholar]
- 19. ) Taketoshi , M. , Yakushiji , T. and Inoue , M.Deoxyribonucleic acid relatedness and phenotypic characteristics of oral Streptococcus milleri strains . Microbios , 73 , 269 – 280 ( 1993. ). [PubMed] [Google Scholar]
- 20. ) Naef , M. , Frei , E. , Soucek , M. , Berthold , M. , Schweizer , W. and Czerniak , A.Surgical‐radiologic interventional treatment in liver abscess with Streptococcus anginosus milleri . Helv. Chir. Acta , 60 , 121 – 125 ( 1993. ). [PubMed] [Google Scholar]