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. 1996 Nov;87(11):1106–1110. doi: 10.1111/j.1349-7006.1996.tb03118.x

Deletion of Src Homology 3 Domain Results in Constitutive Activation of Tec Protein‐Tyrosine Kinase

Yoshihiro Yamashita 1, Akira Miyazato 2, Ken‐ichi Ohya 3, Uichi Ikeda 3, Kazuyuki Shimada 3, Yasusada Miura 2, Keiya Ozawa 1, Hiroyuki Mano 1,✉
PMCID: PMC5921018  PMID: 9045937

Abstract

Tec protein‐tyrosine kinase (PTK) is the prototype of a new subfamily of non‐receptor type PTKs, and is abundantly expressed in hematopoietic tissues. We have revealed that Tec is inducibly tyrosine‐phosphorylated and activated by stimulation with a wide range of cytokines. To get more insight into the signaling mechanism through Tec, we have generated a constitutively active form of Tec PTK. Deletion of the Src homology (SH) 3 domain gave rise to a hyperphosphorylated and activated Tec kinase (TecΔSH3). The activity of TecΔSH3 was confirmed in 293 cells, as well as in cytokine‐dependent hematopoietic cells (BA/F3). TecΔSH3 should be a useful tool to study the in vivo substrates of Tec PTK.

Keywords: Protein‐tyrosine kinase, Tec, Cytokine, SH3

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